Recombinant human 2B4 protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 221 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- Yes
Amino acid sequence
C Q G S A D H V V S I S G V P L Q L Q P N S I Q T K V D S I A W K K L L P S Q N G F H H I L K W E N G S L P S N T S N D R F S F I V K N L S L L I K A A Q Q Q D S G L Y C L E V T S I S G K V Q T A T F Q V F V F D K V E K P R L Q G Q G K I L D R G R C Q V A L S C L V S R D G N V S Y A W Y R G S K L I Q T A G N L T Y L D E E V D I N G T H T Y T C N V S N P V S W E S H T L N L T Q D C Q N A H Q E F R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:11714776, PubMed:8376943). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:11714776, PubMed:8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).
Alternative names
CD244, 2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, SLAM family member 4, Signaling lymphocytic activation molecule 4, NAIL, NKR2B4, h2B4, SLAMF4
Recommended products
Recombinant human 2B4 protein (Fc Chimera Active) is a Human Fragment protein, in the 22 to 221 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, ELISA.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. Immobilized Hab185251 at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag with a linear range of 2-16 ng/mL.
- Accession
- Q9BZW8-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.877% Sodium chloride, 0.75% Glycine, 0.61% Tris, 0.436% L-Arginine
Sequence info
Amino acid sequence
- C Q G S A D H V V S I S G V P L Q L Q P N S I Q T K V D S I A W K K L L P S Q N G F H H I L K W E N G S L P S N T S N D R F S F I V K N L S L L I K A A Q Q Q D S G L Y C L E V T S I S G K V Q T A T F Q V F V F D K V E K P R L Q G Q G K I L D R G R C Q V A L S C L V S R D G N V S Y A W Y R G S K L I Q T A G N L T Y L D E E V D I N G T H T Y T C N V S N P V S W E S H T L N L T Q D C Q N A H Q E F R
- Accession
- Q9BZW8
- Protein length
- Fragment
- Predicted molecular weight
- 48.9 kDa
- Amino acids
- 22 to 221
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:11714776, PubMed:8376943). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:11714776, PubMed:8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).
- Post-translational modifications
N-linked glycosylation is essential for the binding to its ligand CD48 (PubMed:21606496). Also O-glycosylated, in contrast, O-linked sialylation has a negative impact on ligand binding (PubMed:21606496).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon reconstitution add a carrier protein (0.1% BSA)
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
2B4 also known as CD244 or SLAMF4 is a cell surface receptor with a molecular mass of approximately 52-70 kDa. It exists predominantly on various immune cells like natural killer (NK) cells T cells basophils and monocytes. This receptor belongs to the signaling lymphocytic activation molecule (SLAM) family. 2B4's primary role is contributive during cell communication. It specifically recognizes CD48 on neighboring cells and modulates immune responses based on target cell recognition. This function empowers NK cells to maintain immune surveillance and cytotoxicity.
- Biological function summary
2B4 plays an integrative role within cellular immune response. When engaged by its ligand CD48 it regulates activation signals in NK cells and T cells. 2B4 is not a standalone entity but functions as a part of a larger cluster of differentiation molecules. This cluster including CD244.1 and CD244.2 facilitates signal transduction leading to immune modulation. The receptor acts as either an activating or inhibitory agent depending on the expression levels of its associated signaling proteins like SAP (SLAM-associated protein).
- Pathways
Cross-linking of 2B4 forms a critical axis in multiple immune pathways such as the NK cell-mediated cytotoxicity and adaptive immune response pathway. It interacts closely with SAP a protein that modulates downstream signaling events leading to immune cell activation or suppression. Besides SAP 2B4 collaboratively participates with other SLAM family receptors. These interactions optimize the balance between immune activation and tolerance ensuring appropriate immune responses.
- Associated diseases and disorders
2B4 has a significant involvement. Aberrant 2B4 signaling can lead to immune dysregulation linked to diseases like hemophagocytic lymphohistiocytosis (HLH) and certain autoimmune diseases. In HLH defective interactions between 2B4 and SAP contribute to impaired apoptosis of target cells leading to excessive immune proliferation. This dysfunctionality often features alongside compromised activity of related proteins such as perforin which is essential for target cell lysis. Consequently understanding 2B4's function and interaction helps in exploring targeted therapies for immune-associated disorders.
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2 product images
SDS-PAGE - Recombinant human 2B4 protein (Fc Chimera Active) (ab185251)
Human 2B4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 60-80 kDa due to glycosylation.
ELISA - Recombinant human 2B4 protein (Fc Chimera Active) (ab185251)
Immobilized Hab185251 at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag with a linear range of 2-16 ng/mL.
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