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AB183250

Recombinant Human 4E-BP3 protein (His tag N-Terminus)

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Recombinant Human 4E-BP3 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 100 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 3, 4E-BP3, eIF4E-binding protein 3, EIF4EBP3

1 Images
SDS-PAGE - Recombinant Human 4E-BP3 protein (His tag N-Terminus) (AB183250)
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Supplier Data

SDS-PAGE - Recombinant Human 4E-BP3 protein (His tag N-Terminus) (AB183250)

15% SDS PAGE analysis of ab183250 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O60516

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as EIF4EBP3
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSTSTSCPIPGGRDQLPDCYSTTPGGTLYATTPGGTRIIYDRKFLLECKNSPIARTPPCCLPQIPGVTTPPTAPLSKLEELKEQETEEEIPDDAQFEMDI","proteinLength":"Full Length","predictedMolecularWeight":"13.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":100,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O60516","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

4E-BP3 also known as eukaryotic translation initiation factor 4E-binding protein 3 regulates protein synthesis by binding to eukaryotic initiation factor 4E (eIF4E). It is part of the 4E-BP family which also includes 4E-BP1 and 4E-BP2. The molecular weight of 4E-BP3 is approximately 13 kDa. This protein expresses broadly across various tissues with notable expression in the liver and adipose tissues.
Biological function summary

4E-BP3 regulates the initiation of translation by inhibiting eIF4E which is necessary for cap-dependent translation. It acts as a suppressor for protein synthesis during cellular stress or nutrient deprivation. 4E-BP3 does not form a complex with other proteins but functions as part of the mTOR signaling pathway which governs cell growth proliferation and metabolism.

Pathways

4E-BP3 operates within the mTOR signaling pathway a major regulator of cellular growth and metabolism. It plays a role in the regulation of the PI3K/Akt/mTOR pathway. 4E-BP3 directly interacts with eIF4E blocking the formation of the eIF4F complex and inhibiting the translation of specific mRNAs involved in cell growth and survival. The mTOR pathway integrates signals such as nutrients and energy status impacting overall protein homeostasis.

Dysregulation of 4E-BP3 has been linked to conditions like cancer and obesity. In cancer 4E-BP3's malfunction may lead to uncontrolled protein synthesis contributing to tumor growth and progression. Its relationship with mTOR and eIF4E places it among potential targets for cancer therapeutics. In obesity dysfunctional 4E-BP3 expression can affect fat metabolism and energy balance indicating its role in metabolic disorders.
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Specifications

Form

Liquid

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General info

Function

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : the hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repression of translation. In contrast, the hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed : 22684010).

Sequence similarities

Belongs to the eIF4E-binding protein family.

Post-translational modifications

Phosphorylated.

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Product protocols

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Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : the hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repression of translation. In contrast, the hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed : 22684010).
See full target information Eukaryotic translation initiation factor 4E-binding protein 3
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