Recombinant Human 5T4 protein (His tag) is a Human Fragment protein, in the 60 to 345 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
D Q C P A L C E C S E A A R T V K C V N R N L T E V P T D L P A Y V R N L F L T G N Q L A V L P A G A F A R R P P L A E L A A L N L S G S R L D E V R A G A F E H L P S L R Q L D L S H N P L A D L S P F A F S G S N A S V S A P S P L V E L I L N H I V P P E D E R Q N R S F E G M V V A A L L A G R A L Q G L R R L E L A S N H F L Y L P R D V L A Q L P S L R H L D L S N N S L V S L T Y V S F R N L T H L E S L H L E D N A L K V L H N G T L A E L Q G L P H I R V F L D N N P W V C D C H M A D M V T W L K E T E V V Q G K D R L T C A Y P E K M R N R V L L E L N S A D L D C D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Alternative names
5T4, TPBG, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, M6P1, Wnt-activated inhibitory factor 1, 5T4 oncotrophoblast glycoprotein, WAIF1
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Recombinant Human 5T4 protein (His tag) is a Human Fragment protein, in the 60 to 345 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q13641-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- D Q C P A L C E C S E A A R T V K C V N R N L T E V P T D L P A Y V R N L F L T G N Q L A V L P A G A F A R R P P L A E L A A L N L S G S R L D E V R A G A F E H L P S L R Q L D L S H N P L A D L S P F A F S G S N A S V S A P S P L V E L I L N H I V P P E D E R Q N R S F E G M V V A A L L A G R A L Q G L R R L E L A S N H F L Y L P R D V L A Q L P S L R H L D L S N N S L V S L T Y V S F R N L T H L E S L H L E D N A L K V L H N G T L A E L Q G L P H I R V F L D N N P W V C D C H M A D M V T W L K E T E V V Q G K D R L T C A Y P E K M R N R V L L E L N S A D L D C D
- Accession
- Q13641
- Protein length
- Fragment
- Predicted molecular weight
- 33.1 kDa
- Amino acids
- 60 to 345
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
- Post-translational modifications
Highly glycosylated.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5T4 protein also known as trophoblast glycoprotein or B3F1 is a cell surface antigen with a mass of approximately 72 kDa. It is encoded by the TPBG gene and expresses mainly in embryonic tissues and various cancers with limited expression in normal adult tissues. The 5T4 protein is characterized by a densely glycosylated structure contributing to its role in cellular processes like adhesion and migration.
- Biological function summary
5T4 is involved in processes associated with embryonic development and cellular mobility. It contributes to the epithelial-to-mesenchymal transition (EMT) which is a critical process in both normal development and cancer metastasis. Although 5T4 is not part of a known specific complex its glycoprotein nature allows interaction with the extracellular matrix and other cell surface proteins influencing cell behavior and morphology.
- Pathways
5T4 plays a part in signaling pathways involved in cell migration and invasion such as the Wnt/β-catenin pathway and TGF-β signaling. In the context of the Wnt/β-catenin pathway 5T4 interacts with proteins like β-catenin influencing cell proliferation and survival. Through its involvement in TGF-β signaling 5T4 can impact cell differentiation processes and regulatory mechanisms often linking to other proteins such as SMAD family members within these pathways.
- Associated diseases and disorders
5T4 is associated with cancer particularly in its role in promoting metastasis. Its expression is notably increased in several carcinomas including breast and colorectal cancers. The elevated levels of 5T4 in tumors correlate with poorer prognosis making it a potential target for cancer therapies. Additionally 5T4 has connections to proteins such as MMPs (matrix metalloproteinases) which facilitate tissue remodeling and metastatic spread highlighting its significance in cancer progression.
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SDS-PAGE - Recombinant Human 5T4 protein (His tag) (ab276733)
SDS-PAGE analysis of ab276733
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