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AB224840

Recombinant Human 68kDa Neurofilament/NF-L protein

5

(1 Review)

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(6 Publications)

Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) is a Human Full Length protein, in the 2 to 543 aa range with >90% purity and suitable for SDS-PAGE and mass spectrometry. The predicted molecular weight of ab224840 protein is 81.4 kDa.

- Save time and ensure accurate results - use our recombinant NF-L mature protein as a control

View Alternative Names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

2 Images
Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (AB224840)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (AB224840)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.

Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (AB224840)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (AB224840)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P07196

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.158% Tris HCl, 0.029% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant human 68kDa Neurofilament/NF-L protein ab224840 as a positive control in SDS-PAGE and mass spectrometry.

The Neurofilament light polypeptide (NEFL) is labeled with a 10x His-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. It harbors the full length of the mature protein in length.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"MAHHHHHHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGGSHHHHHHHHHHLVPRGSRTSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKDAAAEQKLISEEDL","proteinLength":"Full Length","predictedMolecularWeight":"81.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":543,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07196","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.
Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.
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Specifications

Form

Liquid

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General info

Function

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Sequence similarities

Belongs to the intermediate filament family.

Post-translational modifications

O-glycosylated.. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.. Ubiquitinated in the presence of TRIM2 and UBE2D1.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFL
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Mikrochimica acta 192:131 PubMed39904887

2025

A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein.

Applications

Unspecified application

Species

Unspecified reactive species

Qingting Song,Hailong Zhang,Jia Kong,Man Shing Wong,Hung-Wing Li

Journal of Alzheimer's disease : JAD 96:1623-1638 PubMed38007650

2023

Clinical Evaluation of Cerebrospinal Fluid p217tau and Neurofilament Light Chain Levels in Patients with Alzheimer's Disease or Other Neurological Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Kawarabayashi,Takumi Nakamura,Kazuya Miyashita,Tatsuya Segawa,Isamu Fukamachi,Takashi Sugawara,Hironori Oka,Kunihiko Ishizawa,Masakuni Amari,Hiroo Kasahara,Kouki Makioka,Yoshio Ikeda,Masamitsu Takatama,Mikio Shoji

International journal of molecular sciences 24: PubMed37762074

2023

Plasma Neurofilament Light Chains as Blood-Based Biomarkers for Early Diagnosis of Canine Cognitive Dysfunction Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Chung-Hsin Wu,Xun-Sheng Pan,Li-Yu Su,Shieh-Yueh Yang

ACS measurement science au 2:485-492 PubMed36785659

2022

Development of a 36-Channel Instrument for Assaying Biomarkers of Ultralow Concentrations Utilizing Immunomagnetic Reduction.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Hsien Chen,Ming-Hung Hsu,Kun-Hung Lee,Shieh-Yueh Yang

Frontiers in bioengineering and biotechnology 10:873811 PubMed35402415

2022

Electrochemical Detection of Alzheimer's Disease Biomarker, β-Secretase Enzyme (BACE1), With One-Step Synthesized Reduced Graphene Oxide.

Applications

Unspecified application

Species

Unspecified reactive species

Jhilik Dey,Akanksha Roberts,Subhasis Mahari,Sonu Gandhi,Prem Prakash Tripathi

PloS one 15:e0234519 PubMed32530970

2020

Development of an assay of plasma neurofilament light chain utilizing immunomagnetic reduction technology.

Applications

Unspecified application

Species

Unspecified reactive species

Huei-Chun Liu,Wei-Che Lin,Ming-Jang Chiu,Cheng-Hsien Lu,Chin-Yi Lin,Shieh-Yueh Yang
View all publications
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