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Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) is a Human Full Length protein, in the 2 to 543 aa range with >90% purity and suitable for SDS-PAGE and mass spectrometry. The predicted molecular weight of ab224840 protein is 81.4 kDa.

- Save time and ensure accurate results - use our recombinant NF-L mature protein as a control


Images

Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (AB224840), expandable thumbnail
  • Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (AB224840), expandable thumbnail

Publications

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Tags
His tag N-Terminus
Applications
SDS-PAGE, MS
Biologically active
No

Amino acid sequence

S S F S Y E P Y Y S T S Y K R R Y V E T P R V H I S S V R S G Y S T A R S A Y S S Y S A P V S S S L S V R R S Y S S S S G S L M P S L E N L D L S Q V A A I S N D L K S I R T Q E K A Q L Q D L N D R F A S F I E R V H E L E Q Q N K V L E A E L L V L R Q K H S E P S R F R A L Y E Q E I R D L R L A A E D A T N E K Q A L Q G E R E G L E E T L R N L Q A R Y E E E V L S R E D A E G R L M E A R K G A D E A A L A R A E L E K R I D S L M D E I S F L K K V H E E E I A E L Q A Q I Q Y A Q I S V E M D V T K P D L S A A L K D I R A Q Y E K L A A K N M Q N A E E W F K S R F T V L T E S A A K N T D A V R A A K D E V S E S R R L L K A K T L E I E A C R G M N E A L E K Q L Q E L E D K Q N A D I S A M Q D T I N K L E N E L R T T K S E M A R Y L K E Y Q D L L N V K M A L D I E I A A Y R K L L E G E E T R L S F T S V G S I T S G Y S Q S S Q V F G R S A Y G G L Q T S S Y L M S T R S F P S Y Y T S H V Q E E Q I E V E E T I E A A K A E E A K D E P P S E G E A E E E E K D K E E A E E E E A A E E E E A A K E E S E E A K E E E E G G E G E E G E E T K E A E E E E K K V E G A G E E Q A A K K K D

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

Recommended products

Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) is a Human Full Length protein, in the 2 to 543 aa range with >90% purity and suitable for SDS-PAGE and mass spectrometry. The predicted molecular weight of ab224840 protein is 81.4 kDa.

- Save time and ensure accurate results - use our recombinant NF-L mature protein as a control

Key facts

Purity
>90% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, MS
Accession
P07196-1
Animal free
Yes
Species
Human
Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

S S F S Y E P Y Y S T S Y K R R Y V E T P R V H I S S V R S G Y S T A R S A Y S S Y S A P V S S S L S V R R S Y S S S S G S L M P S L E N L D L S Q V A A I S N D L K S I R T Q E K A Q L Q D L N D R F A S F I E R V H E L E Q Q N K V L E A E L L V L R Q K H S E P S R F R A L Y E Q E I R D L R L A A E D A T N E K Q A L Q G E R E G L E E T L R N L Q A R Y E E E V L S R E D A E G R L M E A R K G A D E A A L A R A E L E K R I D S L M D E I S F L K K V H E E E I A E L Q A Q I Q Y A Q I S V E M D V T K P D L S A A L K D I R A Q Y E K L A A K N M Q N A E E W F K S R F T V L T E S A A K N T D A V R A A K D E V S E S R R L L K A K T L E I E A C R G M N E A L E K Q L Q E L E D K Q N A D I S A M Q D T I N K L E N E L R T T K S E M A R Y L K E Y Q D L L N V K M A L D I E I A A Y R K L L E G E E T R L S F T S V G S I T S G Y S Q S S Q V F G R S A Y G G L Q T S S Y L M S T R S F P S Y Y T S H V Q E E Q I E V E E T I E A A K A E E A K D E P P S E G E A E E E E K D K E E A E E E E A A E E E E A A K E E S E E A K E E E E G G E G E E G E E T K E A E E E E K K V E G A G E E Q A A K K K D
Accession
P07196
Protein length
Full Length
Predicted molecular weight
81.4 kDa
Amino acids
2 to 543
Nature
Recombinant
Tags
His tag N-Terminus

Specifications

Form
Liquid

General info

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Sequence similarities

Belongs to the intermediate filament family.

Post-translational modifications

O-glycosylated.

Subcellular localisation
Cytoskeleton

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Ensure the validity of your result using our recombinant human 68kDa Neurofilament/NF-L protein ab224840 as a positive control in SDS-PAGE and mass spectrometry.

The Neurofilament light polypeptide (NEFL) is labeled with a 10x His-SUMO-tag at the N-terminus and a Myc-tag at the C-terminus. It harbors the full length of the mature protein in length.


Check out our protein gel staining guide for SDS-PAGE here

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Associated diseases and disorders

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

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2 product images

  • Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (ab224840), expandable thumbnail

    Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (ab224840)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.

  • Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (ab224840), expandable thumbnail

    Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (His tag) (ab224840)

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab224840 could indicate that this peptide derived from E.coli-expressed human Neurofilament protein.

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Product protocols

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