Recombinant Human ABCA1 protein is a Human Fragment protein, in the 2085 to 2245 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Publications
Filter by
- Scientific reports 8:110612018Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLimei Wang,Pierre Eftekhari,Daniel Schachner,Irena D Ignatova,Veronika Palme,Nicole Schilcher,Angela Ladurner,Elke H Heiss,Herbert Stangl,Verena M Dirsch,Atanas G AtanasovPubMed 30038271
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
N C A L S V V K E G R S V V L T S H S M E E C E A L C T R M A I M V N G R F R C L G S V Q H L K N R F G D G Y T I V V R I A G S N P D L K P V Q D F F G L A F P G S V L K E K H R N M L Q Y Q L P S S L S S L A R I F S I L S Q S K K R L H I E D Y S V S Q T T L D Q V F V N F A K D Q S D D D H L K D L S L H K N Q T V V D V A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
Alternative names
ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1
Recommended products
Recombinant Human ABCA1 protein is a Human Fragment protein, in the 2085 to 2245 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O95477-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
Constituents: 0.58% Sodium chloride, 0.32% Tris HCl, 0.2% Guanidine hydrochloride
Sequence info
Amino acid sequence
- N C A L S V V K E G R S V V L T S H S M E E C E A L C T R M A I M V N G R F R C L G S V Q H L K N R F G D G Y T I V V R I A G S N P D L K P V Q D F F G L A F P G S V L K E K H R N M L Q Y Q L P S S L S S L A R I F S I L S Q S K K R L H I E D Y S V S Q T T L D Q V F V N F A K D Q S D D D H L K D L S L H K N Q T V V D V A
- Accession
- O95477
- Protein length
- Fragment
- Amino acids
- 2085 to 2245
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purified via His tag
General info
- Function
Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981, PubMed:35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed:14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed:24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed:10533863, PubMed:14754908, PubMed:24097981, PubMed:35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed:35974019).
- Sequence similarities
Belongs to the ABC transporter superfamily. ABCA family.
- Post-translational modifications
Phosphorylation on Ser-2054 regulates phospholipid efflux.
- Subcellular localisation
- Endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
- Biological function summary
ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.
- Pathways
Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.
- Associated diseases and disorders
ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human ABCA1 protein (ab125995)
SDS PAGE of ab125995 at 0.5µg loaded and stained with Coomassie Brilliant Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com