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AB125995

Recombinant Human ABCA1 protein (His-DHFR)

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(1 Publication)

Recombinant Human ABCA1 protein (His-DHFR) is a Human Fragment protein, in the 2085 to 2245 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ABC1, CERP, ABCA1, Phospholipid-transporting ATPase ABCA1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, Cholesterol efflux regulatory protein, ABC-1, ATP-binding cassette 1

1 Images
SDS-PAGE - Recombinant Human ABCA1 protein (His-DHFR) (AB125995)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ABCA1 protein (His-DHFR) (AB125995)

SDS PAGE of ab125995 at 0.5µg loaded and stained with Coomassie Brilliant Blue.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His-DHFR tag

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O95477

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.58% Sodium chloride, 0.32% Tris HCl, 0.2% Guanidine hydrochloride

storage-buffer

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Complementary Products

Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
Primary Antibodies

AB52617

Anti-ABCG1 antibody [EP1366Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ABCG1 antibody [EP1366Y] (AB52617)

  • 4
  • 5
Primary Antibodies

AB223056

Anti-ABCG8 antibody

Western blot - Anti-ABCG8 antibody (AB223056)

  • 0
  • 0
Primary Antibodies

AB32577

Anti-Caveolin-1 antibody [E249] - Caveolae Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (AB32577)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVA","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":2245,"aminoAcidStart":2085,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95477","tags":[{"tag":"His-DHFR","terminus":""}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.
Biological function summary

ABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.

Pathways

Many regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.

ABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.
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Specifications

Form

Lyophilized

Additional notes

Purified via His tag

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General info

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed : 24097981, PubMed : 35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed : 14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed : 24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed : 10533863, PubMed : 14754908, PubMed : 24097981, PubMed : 35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed : 35974019).

Sequence similarities

Belongs to the ABC transporter superfamily. ABCA family.

Post-translational modifications

Phosphorylation on Ser-2054 regulates phospholipid efflux.. Palmitoylated by ZDHHC8 (PubMed:19556522). Palmitoylation is essential for localization to the plasma membrane (PubMed:19556522).

Subcellular localisation

Endosome

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Product protocols

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Target data

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP (PubMed : 24097981, PubMed : 35974019). Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs (PubMed : 14754908). Transports preferentially phosphatidylcholine over phosphatidylserine (PubMed : 24097981). May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs (PubMed : 10533863, PubMed : 14754908, PubMed : 24097981, PubMed : 35974019). Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain (PubMed : 35974019).
See full target information ABCA1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 8:11061 PubMed30038271

2018

Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Wang,Pierre Eftekhari,Daniel Schachner,Irena D Ignatova,Veronika Palme,Nicole Schilcher,Angela Ladurner,Elke H Heiss,Herbert Stangl,Verena M Dirsch,Atanas G Atanasov
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Product promise

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