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AB314521

Recombinant human ABCB8 protein (His tag)

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Recombinant human ABCB8 protein (His tag) is a Human Fragment protein, in the 38 to 693 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

MABC1, MITOSUR, ABCB8, Mitochondrial potassium channel ATP-binding subunit, Mitochondrial ATP-binding cassette 1, Mitochondrial sulfonylurea-receptor, M-ABC1

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SDS-PAGE - Recombinant human ABCB8 protein (His tag) (AB314521)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ABCB8 protein (His tag) (AB314521)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NUT2

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.14% PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"GKTGQLHKAEGEYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTH","proteinLength":"Fragment","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":693,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NUT2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target ABCB8 also known as ATP-binding cassette sub-family B member 8 is a mitochondrial membrane protein. It has a molecular mass of approximately 82 kDa. ABCB8 is expressed in multiple tissues with a higher presence noted in the heart and skeletal muscle. It functions as a transporter protein involved in the translocation of molecules across intracellular membranes particularly in the mitochondria.
Biological function summary

ABCB8 plays a significant role in maintaining mitochondrial homeostasis and cellular iron metabolism. It forms part of a complex with several other proteins that are essential for mitochondrial function. ABCB8 helps in the export of iron-sulfur clusters from the mitochondria to the cytosol which is critical for various cellular processes.

Pathways

ABCB8 integrates into cellular pathways focused on iron-sulfur cluster biosynthesis and export. It interacts with proteins involved in the mitochondrial iron homeostasis pathway such as frataxin and ABCB7. These interactions underline its involvement in key processes that regulate cellular iron distribution and oxidative protection.

Impairments in ABCB8 function have links to conditions like cardiomyopathy and neurodegeneration. Direct connection to proteins such as ABCB7 can exacerbate these disorders when ABCB8 does not function correctly. This connection suggests a pivotal role for ABCB8 in maintaining cellular health and preventing mitochondrial-related diseases.
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Specifications

Form

Lyophilized

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General info

Function

ATP-binding subunit of the mitochondrial ATP-gated potassium channel (mitoK(ATP)) (PubMed : 31435016). Together with pore-forming subunit CCDC51/MITOK of the mitoK(ATP) channel, mediates ATP-dependent potassium currents across the mitochondrial inner membrane (PubMed : 31435016). An increase in ATP intracellular levels closes the channel, inhibiting K(+) transport, whereas a decrease in ATP levels enhances K(+) uptake in the mitochondrial matrix (PubMed : 31435016). Plays a role in mitochondrial iron transport (PubMed : 30623799). Required for maintenance of normal cardiac function, possibly by influencing mitochondrial iron export and regulating the maturation of cytosolic iron sulfur cluster-containing enzymes (By similarity).

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Subcellular localisation

Mitochondrion inner membrane

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Product protocols

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Target data

ATP-binding subunit of the mitochondrial ATP-gated potassium channel (mitoK(ATP)) (PubMed : 31435016). Together with pore-forming subunit CCDC51/MITOK of the mitoK(ATP) channel, mediates ATP-dependent potassium currents across the mitochondrial inner membrane (PubMed : 31435016). An increase in ATP intracellular levels closes the channel, inhibiting K(+) transport, whereas a decrease in ATP levels enhances K(+) uptake in the mitochondrial matrix (PubMed : 31435016). Plays a role in mitochondrial iron transport (PubMed : 30623799). Required for maintenance of normal cardiac function, possibly by influencing mitochondrial iron export and regulating the maturation of cytosolic iron sulfur cluster-containing enzymes (By similarity).
See full target information ABCB8
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Complementary Products

Primary Antibodies

AB104498

Anti-ABCB8 antibody

Western blot - Anti-ABCB8 antibody (AB104498)

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