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AB314519

Recombinant human ABCD2 protein (His tag)

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Recombinant human ABCD2 protein (His tag) is a Human Full Length protein, in the 1 to 740 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ALD1, ALDL1, ALDR, ALDRP, ABCD2, ATP-binding cassette sub-family D member 2, Adrenoleukodystrophy-like 1, Adrenoleukodystrophy-related protein, hALDR

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SDS-PAGE - Recombinant human ABCD2 protein (His tag) (AB314519)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human ABCD2 protein (His tag) (AB314519)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9UBJ2

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol

storage-buffer

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Complementary Products

Primary Antibodies

AB97383

Anti-ABCD2 antibody

Western blot - Anti-ABCD2 antibody (AB97383)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTETICEKPSPGVNADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGKLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKSYKALADQMNLILSKRLWYIMIEQFLMKYVWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS","proteinLength":"Full Length","predictedMolecularWeight":"89.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":740,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UBJ2","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ABCD2 protein also known as ALDR (adrenoleukodystrophy-related protein) is a member of the ATP-binding cassette (ABC) transporter family located on the peroxisomal membrane. It plays a role in the transport of very-long-chain fatty acids (VLCFAs) into peroxisomes. The molecular mass of ABCD2 is approximately 80 kDa. Expression of ABCD2 occurs widely in tissues with high levels found in adrenal glands brain and liver.
Biological function summary

ABCD2 influences the metabolism of lipids within cells by facilitating the import of VLCFAs which are subsequently broken down through beta-oxidation inside peroxisomes. It operates as part of a peroxisomal beta-oxidation complex. This protein also shares functional similarities with other ABC family transporters such as ABCD1 though with slightly different substrate specificities. Expression of ABCD2 increases in response to certain dietary and hormonal factors indicating a role in metabolic adaptation.

Pathways

ABCD2 integrates into peroxisomal fatty acid beta-oxidation and lipid metabolism pathways. It associates closely with ABCD1 within these pathways contributing to the regulation of fatty acid levels in cells. A deficiency in ABCD2 affects these metabolic pathways by altering VLCFA transport and utilization leading to an imbalance with downstream metabolic effects. The protein also interacts with PPAR (peroxisome proliferator-activated receptors) signaling influencing lipid homeostasis.

Issues with ABCD2 are linked to adrenoleukodystrophy (ALD) an inherited disorder that affects the nervous system and adrenal glands due to the accumulation of VLCFAs. The disease connects ABCD2 and ABCD1 proteins since mutations in the ABCD1 gene are known to cause ALD. Researchers are interested in ABCD2's potential compensatory role in similar metabolic pathways possibly offering therapeutic implications. Recent studies also explore connections between ABCD2 dysregulation and liver disorders involving VLCFA metabolism but further research is necessary to establish these links conclusively.
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Specifications

Form

Lyophilized

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General info

Function

ATP-dependent transporter of the ATP-binding cassette (ABC) family involved in the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen (PubMed : 21145416, PubMed : 29397936). Like ABCD1 seems to have fatty acyl-CoA thioesterase (ACOT) and ATPase activities, according to this model, VLCFA-CoA as free VLCFA is transpoted in an ATP-dependent manner into peroxisomes after the hydrolysis of VLCFA-CoA mediated by the ACOT activity of ABCD2 (Probable) (PubMed : 29397936). Shows overlapping substrate specificities with ABCD1 toward saturated fatty acids (FA) and monounsaturated FA (MUFA) but has a distinct substrate preference for shorter VLCFA (C22 : 0) and polyunsaturated fatty acid (PUFA) such as C22 : 6-CoA and C24 : 6-CoA (in vitro) (PubMed : 21145416). Thus, may play a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed : 21145416).

Sequence similarities

Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.

Subcellular localisation

Peroxisome membrane

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Product protocols

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Target data

ATP-dependent transporter of the ATP-binding cassette (ABC) family involved in the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen (PubMed : 21145416, PubMed : 29397936). Like ABCD1 seems to have fatty acyl-CoA thioesterase (ACOT) and ATPase activities, according to this model, VLCFA-CoA as free VLCFA is transpoted in an ATP-dependent manner into peroxisomes after the hydrolysis of VLCFA-CoA mediated by the ACOT activity of ABCD2 (Probable) (PubMed : 29397936). Shows overlapping substrate specificities with ABCD1 toward saturated fatty acids (FA) and monounsaturated FA (MUFA) but has a distinct substrate preference for shorter VLCFA (C22 : 0) and polyunsaturated fatty acid (PUFA) such as C22 : 6-CoA and C24 : 6-CoA (in vitro) (PubMed : 21145416). Thus, may play a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation (PubMed : 21145416).
See full target information ABCD2
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