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AB123190

Recombinant Human ABHD14B protein (His tag N-Terminus)

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Recombinant Human ABHD14B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 210 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CIB, ABHD14B, Putative protein-lysine deacylase ABHD14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, Abhydrolase domain-containing protein 14B

1 Images
SDS-PAGE - Recombinant Human ABHD14B protein (His tag N-Terminus) (AB123190)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ABHD14B protein (His tag N-Terminus) (AB123190)

SDS-PAGE analysis of Recombinant Human ABHD14B protein (ab123190), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human ABHD14B protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96IU4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQAGYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPFLTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ","proteinLength":"Full Length","predictedMolecularWeight":"25 kDa","actualMolecularWeight":null,"aminoAcidEnd":210,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96IU4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ABHD14B also known as abhydrolase domain containing 14B has a significant role in biochemical processes. It is about 42 kDa in mass. The ABHD14B protein functions as a serine hydrolase which means it belongs to a broad category of enzymes that hydrolyze ester bonds. It is expressed mostly in the liver and brain indicating its roles in various cellular functions in these tissues.
Biological function summary

ABHD14B plays a part in cellular metabolism and signal transduction. It interacts with other proteins within a multi-protein complex to regulate lipid homeostasis influencing the production and breakdown of lipid molecules. ABHD14B enzymes modulate these processes which are essential for maintaining cell membrane integrity and signaling events that control cell growth and differentiation.

Pathways

The involvement of ABHD14B includes the lipid signaling and metabolic regulation pathways. It has connections with the fatty acid metabolism pathway coordinating with proteins like ACC and FASN to manage fatty acid synthesis and degradation. Its activities impact lipid synthesis and degradation showing its importance in maintaining cellular energy balance and signaling.

Improper function of ABHD14B links to metabolic diseases such as hepatic steatosis also known as fatty liver disease. It also holds connections to neurological disorders owing to its expression in the brain. Proteins involved in these conditions like ACC in metabolic disorders or Tau in neurological diseases interact with ABHD14B indicating its potential role in these pathologies. Understanding these interactions may provide insights into therapeutic targets.
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Specifications

Form

Liquid

Additional notes

ab123190 is purified using conventional chromatography techniques.

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General info

Function

Acts as an atypical protein-lysine deacetylase in vitro (PubMed : 31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed : 31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed : 14672934, PubMed : 31478652). May activate transcription (PubMed : 14672934).

Sequence similarities

Belongs to the AB hydrolase superfamily. ABHD14 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as an atypical protein-lysine deacetylase in vitro (PubMed : 31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed : 31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed : 14672934, PubMed : 31478652). May activate transcription (PubMed : 14672934).
See full target information ABHD14B
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