Recombinant Human Abhd5/CGI-58 protein is a Human Full Length protein, in the 1 to 349 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A E E E E V D S A D T G E R S G W L T G W L P T W C P T S I S H L K E A E E K M L K C V P C T Y K K E P V R I S N G N K I W T L K F S H N I S N K T P L V L L H G F G G G L G L W A L N F G D L C T N R P V Y A F D L L G F G R S S R P R F D S D A E E V E N Q F V E S I E E W R C A L G L D K M I L L G H N L G G F L A A A Y S L K Y P S R V N H L I L V E P W G F P E R P D L A D Q D R P I P V W I R A L G A A L T P F N P L A G L R I A G P F G L S L V Q R L R P D F K R K Y S S M F E D D T V T E Y I Y H C N V Q T P S G E T A F K N M T I P Y G W A K R P M L Q R I G K M H P D I P V S V I F G A R S C I D G N S G T S I Q S L R P H S Y V K T I A I L G A G H Y V Y A D Q P E E F N Q K V K E I C D T V D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed:18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion (By similarity).
Alternative names
NCIE2, CGI-58, ABHD5, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, Lipid droplet-binding protein CGI-58
Recommended products
Recombinant Human Abhd5/CGI-58 protein is a Human Full Length protein, in the 1 to 349 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q8WTS1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A A E E E E V D S A D T G E R S G W L T G W L P T W C P T S I S H L K E A E E K M L K C V P C T Y K K E P V R I S N G N K I W T L K F S H N I S N K T P L V L L H G F G G G L G L W A L N F G D L C T N R P V Y A F D L L G F G R S S R P R F D S D A E E V E N Q F V E S I E E W R C A L G L D K M I L L G H N L G G F L A A A Y S L K Y P S R V N H L I L V E P W G F P E R P D L A D Q D R P I P V W I R A L G A A L T P F N P L A G L R I A G P F G L S L V Q R L R P D F K R K Y S S M F E D D T V T E Y I Y H C N V Q T P S G E T A F K N M T I P Y G W A K R P M L Q R I G K M H P D I P V S V I F G A R S C I D G N S G T S I Q S L R P H S Y V K T I A I L G A G H Y V Y A D Q P E E F N Q K V K E I C D T V D
- Accession
- Q8WTS1
- Protein length
- Full Length
- Predicted molecular weight
- 64.13 kDa
- Amino acids
- 1 to 349
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid (PubMed:18606822). Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis (PubMed:18606822). May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2 (PubMed:16679289). Involved in keratinocyte differentiation (PubMed:18832586). Regulates lipid droplet fusion (By similarity).
- Sequence similarities
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Abhd5.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Abhd5 also known as CGI-58 is a protein with significant importance in lipid metabolism. It has a molecular weight of approximately 39 kDa and is ubiquitously expressed in tissues throughout the body including adipose tissue and liver. This protein associates with lipid droplets within cells serving a role important for lipid handling and signaling. Abhd5 stands for the alpha/beta hydrolase domain-containing protein 5 indicative of its structure and enzymatic functions.
- Biological function summary
The Abhd5/CGI-58 protein acts as a coactivator of adipose triglyceride lipase (ATGL) enhancing its role in triglyceride hydrolysis. This support is vital for energy release from triglycerides in fat cells. Abhd5 does not function alone; it exists within a protein complex where it modulates ATGL's catalytic activity. Furthermore Abhd5 is implicated in broader lipid signaling and metabolic control influencing various cellular responses through its roles within these complexes.
- Pathways
Abhd5/CGI-58 is central to the lipid metabolism pathway particularly in the breakdown of triglycerides. By activating ATGL Abhd5 influences the lipolytic pathway which is important for mobilizing stored fats for energy use. In addition to its role with ATGL another protein perilipin regulates this pathway by interacting with lipid droplet-associated proteins including Abhd5 to control access to lipids. The protein influences the signaling crosstalk within metabolic pathways coordinating metabolic responses within the cell.
- Associated diseases and disorders
The Abhd5/CGI-58 protein has ties to conditions like lipodystrophy and Chanarin-Dorfman syndrome. Deficiencies or mutations in the CGI-58 protein can impair lipid metabolism leading to abnormal fat distribution and storage. In these diseases the lack of Abhd5 activity affects the interactions with proteins like ATGL which is responsible for triglyceride breakdown. Research continues to explore the relationship between Abhd5 dysfunction and these metabolic disorders aiming to clarify its role in the etiology of such conditions.
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1 product image
SDS-PAGE - Recombinant Human Abhd5/CGI-58 protein (ab132557)
12.5% SDS-PAGE analysis of ab132557 stained with Coomassie Blue.
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