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AB323060

Recombinant Human ABP1 Protein Standard

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Recombinant Human ABP1 Protein Standard is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

View Alternative Names

ABP, ABP1, DAO, DAO1, AOC1, Diamine oxidase [copper-containing], Diamine oxidase, Amiloride-binding protein, Amiloride-binding protein 1, Amine oxidase copper domain-containing protein 1, Histaminase, Kidney amine oxidase, KAO, KDAO

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Sandwich ELISA - Recombinant Human ABP1 Protein Standard (AB323060)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human ABP1 Protein Standard (AB323060)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human ABP1 Protein Standard (AB323060)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ABP1 Protein Standard (AB323060)

SDS-PAGE analysis of ab323060 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P19801

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"84.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P19801","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 12072962, PubMed : 19764817, PubMed : 239684, PubMed : 8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed : 12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed : 12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed : 12072962, PubMed : 239684).

Sequence similarities

Belongs to the copper/topaquinone oxidase family.

Post-translational modifications

N-glycosylated.. Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.

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Product protocols

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Target data

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 12072962, PubMed : 19764817, PubMed : 239684, PubMed : 8144586). Its preferred substrates are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses (PubMed : 12072962). Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism (PubMed : 12072962). It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes (PubMed : 12072962, PubMed : 239684).
See full target information AOC1
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Product promise

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