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AB98236

Recombinant Human ACAA1/Beta-ketothiolase protein

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Recombinant Human ACAA1/Beta-ketothiolase protein is a Human Full Length protein, in the 27 to 424 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ACAA, PTHIO, ACAA1, Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Beta-ketothiolase, Peroxisomal 3-oxoacyl-CoA thiolase

1 Images
SDS-PAGE - Recombinant Human ACAA1/Beta-ketothiolase protein (AB98236)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ACAA1/Beta-ketothiolase protein (AB98236)

15% SDS-PAGE analysis of 3µg ab98236.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P09110

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ACAA1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLSGAPQASAADVVVVHGRRTAICRAGRGGFKDTTPDELLSAVMTAVLKDVNLRPEQLGDICVGNVLQPGAGAIMARIAQFLSDIPETVPLSTVNRQCSSGLQAVASIAGGIRNGSYDIGMACGVESMSLADRGNPGNITSRLMEKEKARDCLIPMGITSENVAERFGISREKQDTFALASQQKAARAQSKGCFQAEIVPVTTTVHDDKGTKRSITVTQDEGIRPSTTMEGLAKLKPAFKKDGSTTAGNSSQVSDGAAAILLARRSKAEELGLPILGVLRSYAVVGVPPDIMGIGPAYAIPVALQKAGLTVSDVDIFEINEAFASQAAYCVEKLRLPPEKVNPLGGAVALGHPLGCTGARQVITLLNELKRRGKRAYGVVSMCIGTGMGAAAVFEYPGN","proteinLength":"Full Length","predictedMolecularWeight":"43.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":424,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09110","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACAA1 also known as Beta-ketothiolase is an enzyme involved in key metabolic processes. This enzyme with a molecular mass of approximately 41 kDa plays a pivotal role in the thiolytic cleavage of 3-ketoacyl-CoA an essential step in both fatty acid beta-oxidation and ketogenesis. ACAA1 is expressed in various tissues notably liver and muscle where high rates of fatty acid metabolism occur. As part of the peroxisome it functions effectively to maintain cellular lipid homeostasis.
Biological function summary

ACAA1 facilitates the metabolic breakdown of fatty acids by participating in the final steps of beta-oxidation. It does not form part of a larger enzymatic complex but it operates alongside enzymes like acyl-CoA oxidase and enoyl-CoA hydratase that contribute to the fatty acid oxidation pathway. Through its enzymatic activity ACAA1 directly influences energy production and metabolic rate which are important for maintaining cellular energy balance.

Pathways

Beta-ketothiolase plays an integral role in the fatty acid beta-oxidation and ketogenesis pathways. These pathways are critical for the breakdown of fatty acids into acetyl-CoA which enters the citric acid cycle for energy production. ACAA1 works closely with enzymes such as acetoacetyl-CoA thiolase and HMG-CoA lyase in these processes highlighting its involvement in converting fatty acids into usable energy forms.

Malfunction or deficiency in ACAA1 can lead to issues like ketoacidosis and metabolic dysregulation disorders. The enzyme's dysfunction can result in improper fatty acid metabolism and accumulation of toxic intermediates. ACAA1 shares a connection with HMG-CoA synthase in ketogenesis and any disruption in these pathways may contribute to metabolic diseases impacting energy homeostasis and leading to serious health conditions.
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Specifications

Form

Liquid

Additional notes

ab98236 was purified using conventional chromatography techniques.

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General info

Function

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).

Sequence similarities

Belongs to the thiolase-like superfamily. Thiolase family.

Subcellular localisation

Peroxisome

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Product protocols

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Target data

Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (PubMed : 11734571, PubMed : 2882519). Plays an important role in fatty acid peroxisomal beta-oxidation (PubMed : 11734571, PubMed : 2882519). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs (PubMed : 11734571, PubMed : 2882519).
See full target information ACAA1
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