Recombinant Human ACADL/LCAD protein is a Human Full Length protein, in the 1 to 430 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A R L L R G S L R V L G G H R A P R Q L P A A R C S H S G G E E R L E T P S A K K L T D I G I R R I F S P E H D I F R K S V R K F F Q E E V I P H H S E W E K A G E V S R E V W E K A G K Q G L L G V N I A E H L G G I G G D L Y S A A I V W E E Q A Y S N C S G P G F S I H S G I V M S Y I T N H G S E E Q I K H F I P Q M T A G K C I G A I A M T E P G A G S D L Q G I K T N A K K D G S D W I L N G S K V F I S N G S L S D V V I V V A V T N H E A P S P A H G I S L F L V E N G M K G F I K G R K L H K M G L K A Q D T A E L F F E D I R L P A S A L L G E E N K G F Y Y I M K E L P Q E R L L I A D V A I S A S E F M F E E T R N Y V K Q R K A F G K T V A H L Q T V Q H K L A E L K T H I C V T R A F V D N C L Q L H E A K R L D S A T A C M A K Y W A S E L Q N S V A Y D C V Q L H G G W G Y M W E Y P I A K A Y V D A R V Q P I Y G G T N E I M K E L I A R E I V F D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Associated Products
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Target data
Function
Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed:21237683, PubMed:8823175).
Alternative names
LCAD, ACADL
Recommended products
Recombinant Human ACADL/LCAD protein is a Human Full Length protein, in the 1 to 430 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P28330-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A A R L L R G S L R V L G G H R A P R Q L P A A R C S H S G G E E R L E T P S A K K L T D I G I R R I F S P E H D I F R K S V R K F F Q E E V I P H H S E W E K A G E V S R E V W E K A G K Q G L L G V N I A E H L G G I G G D L Y S A A I V W E E Q A Y S N C S G P G F S I H S G I V M S Y I T N H G S E E Q I K H F I P Q M T A G K C I G A I A M T E P G A G S D L Q G I K T N A K K D G S D W I L N G S K V F I S N G S L S D V V I V V A V T N H E A P S P A H G I S L F L V E N G M K G F I K G R K L H K M G L K A Q D T A E L F F E D I R L P A S A L L G E E N K G F Y Y I M K E L P Q E R L L I A D V A I S A S E F M F E E T R N Y V K Q R K A F G K T V A H L Q T V Q H K L A E L K T H I C V T R A F V D N C L Q L H E A K R L D S A T A C M A K Y W A S E L Q N S V A Y D C V Q L H G G W G Y M W E Y P I A K A Y V D A R V Q P I Y G G T N E I M K E L I A R E I V F D K
- Accession
- P28330
- Protein length
- Full Length
- Predicted molecular weight
- 73.41 kDa
- Amino acids
- 1 to 430
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed:21237683, PubMed:8823175).
- Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
- Post-translational modifications
Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as ACADL.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ACADL also known as LCAD (Long-Chain Acyl-CoA Dehydrogenase) is an enzyme important for fatty acid metabolism. It has a molecular mass of approximately 43 kDa. This protein is mainly found in mitochondria and you can find high expression levels in tissues with significant energy demands like liver muscle and heart. The mechanical function involves catalyzing the initial step in the beta-oxidation cycle which is the oxidation of long-chain acyl-CoA substrates to form a trans-double bond between the beta and alpha carbons.
- Biological function summary
ACADL plays an essential role in mitochondrial energy production. It is part of the electron transport chain more specifically involved in the oxidation of fatty acids. This process is central to sustaining energy and heat production especially during fasting. ACADL works as part of a multi-enzyme complex known as the mitochondrial trifunctional protein complex responsible for multiple steps in fatty acid oxidation.
- Pathways
ACADL's function in beta-oxidation links it to key metabolic pathways like energy metabolism and fatty acid catabolism. It directly interacts and works with other proteins such as enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase which facilitate subsequent steps after ACADL's initial action in the same beta-oxidation pathway. This integration ensures the proper breakdown of fatty acids to acetyl-CoA feeding into the citric acid cycle for ATP production.
- Associated diseases and disorders
Defects in ACADL activity are implicate in metabolic conditions such as long-chain acyl-CoA dehydrogenase deficiency (LCADD) and related cardiomyopathies. LCADD can lead to severe symptoms involving muscle weakness cardiomyopathy and hypoglycemia. ACADL's role in these disorders often intersects with other proteins like VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) since deficiencies or dysfunctions can manifest in overlapping symptoms due to their shared role in fatty acid oxidation.
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1 product image
SDS-PAGE - Recombinant Human ACADL/LCAD protein (ab114591)
12.5% SDS-PAGE showing ab114591 at approximately 73.41kDa stained with Coomassie Blue.
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