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AB114591

Recombinant Human ACADL/LCAD protein (GST tag N-Terminus)

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Recombinant Human ACADL/LCAD protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 430 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

LCAD, ACADL

1 Images
SDS-PAGE - Recombinant Human ACADL/LCAD protein (GST tag N-Terminus) (AB114591)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ACADL/LCAD protein (GST tag N-Terminus) (AB114591)

12.5% SDS-PAGE showing ab114591 at approximately 73.41kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P28330

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB128568

Anti-CPT1A antibody [8F6AE9]

BOND RX™ Validated|KO Validated

Western blot - Anti-CPT1A antibody [8F6AE9] (AB128568)

  • 5
  • 5
Primary Antibodies

AB181114

Anti-CPT2 antibody [EPR13626] - C-terminal

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CPT2 antibody [EPR13626] - C-terminal (AB181114)

  • 2
  • 1
Primary Antibodies

AB72230

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein).</p>" } } }
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Product details

This product was previously labelled as ACADL.
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Sequence info

[{"sequence":"MAARLLRGSLRVLGGHRAPRQLPAARCSHSGGEERLETPSAKKLTDIGIRRIFSPEHDIFRKSVRKFFQEEVIPHHSEWEKAGEVSREVWEKAGKQGLLGVNIAEHLGGIGGDLYSAAIVWEEQAYSNCSGPGFSIHSGIVMSYITNHGSEEQIKHFIPQMTAGKCIGAIAMTEPGAGSDLQGIKTNAKKDGSDWILNGSKVFISNGSLSDVVIVVAVTNHEAPSPAHGISLFLVENGMKGFIKGRKLHKMGLKAQDTAELFFEDIRLPASALLGEENKGFYYIMKELPQERLLIADVAISASEFMFEETRNYVKQRKAFGKTVAHLQTVQHKLAELKTHICVTRAFVDNCLQLHEAKRLDSATACMAKYWASELQNSVAYDCVQLHGGWGYMWEYPIAKAYVDARVQPIYGGTNEIMKELIAREIVFDK","proteinLength":"Full Length","predictedMolecularWeight":"73.41 kDa","actualMolecularWeight":null,"aminoAcidEnd":430,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P28330","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACADL also known as LCAD (Long-Chain Acyl-CoA Dehydrogenase) is an enzyme important for fatty acid metabolism. It has a molecular mass of approximately 43 kDa. This protein is mainly found in mitochondria and you can find high expression levels in tissues with significant energy demands like liver muscle and heart. The mechanical function involves catalyzing the initial step in the beta-oxidation cycle which is the oxidation of long-chain acyl-CoA substrates to form a trans-double bond between the beta and alpha carbons.
Biological function summary

ACADL plays an essential role in mitochondrial energy production. It is part of the electron transport chain more specifically involved in the oxidation of fatty acids. This process is central to sustaining energy and heat production especially during fasting. ACADL works as part of a multi-enzyme complex known as the mitochondrial trifunctional protein complex responsible for multiple steps in fatty acid oxidation.

Pathways

ACADL's function in beta-oxidation links it to key metabolic pathways like energy metabolism and fatty acid catabolism. It directly interacts and works with other proteins such as enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase which facilitate subsequent steps after ACADL's initial action in the same beta-oxidation pathway. This integration ensures the proper breakdown of fatty acids to acetyl-CoA feeding into the citric acid cycle for ATP production.

Defects in ACADL activity are implicate in metabolic conditions such as long-chain acyl-CoA dehydrogenase deficiency (LCADD) and related cardiomyopathies. LCADD can lead to severe symptoms involving muscle weakness cardiomyopathy and hypoglycemia. ACADL's role in these disorders often intersects with other proteins like VLCAD (Very Long-Chain Acyl-CoA Dehydrogenase) since deficiencies or dysfunctions can manifest in overlapping symptoms due to their shared role in fatty acid oxidation.
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Specifications

Form

Liquid

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General info

Function

Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 24591516, PubMed : 32389575). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 17564966, PubMed : 21237683). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase activity overlaps with that of ACADV and ACAD9, acting on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 8823175). Plays a primary role in FAO in tissues where it is the main long-chain ACAD expressed, such as the lung, specifically in type 2 alveolar cells (responsible for surfactant production) (PubMed : 17564966, PubMed : 24591516). Probably responsible for beta-oxidation of bulky substrates including branched chain fatty acyl-CoAs and sterol derivatives thanks to its enlarged substrate-binding cavity (PubMed : 38839792).

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Post-translational modifications

Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed : 17564966, PubMed : 24591516, PubMed : 32389575). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed : 17564966, PubMed : 21237683). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase activity overlaps with that of ACADV and ACAD9, acting on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains (PubMed : 17564966, PubMed : 21237683, PubMed : 8823175). Plays a primary role in FAO in tissues where it is the main long-chain ACAD expressed, such as the lung, specifically in type 2 alveolar cells (responsible for surfactant production) (PubMed : 17564966, PubMed : 24591516). Probably responsible for beta-oxidation of bulky substrates including branched chain fatty acyl-CoAs and sterol derivatives thanks to its enlarged substrate-binding cavity (PubMed : 38839792).
See full target information ACADL
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