Recombinant Human ACADM/MCAD protein is a Human Full Length protein, in the 26 to 421 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, HPLC
No
K A N R Q R E P G L G F S F E F T E Q Q K E F Q A T A R K F A R E E I I P V A A E Y D K T G E Y P V P L I R R A W E L G L M N T H I P E N C G G L G L G T F D A C L I S E E L A Y G C T G V Q T A I E G N S L G Q M P I I I A G N D Q Q K K K Y L G R M T E E P L M C A Y C V T E P G A G S D V A G I K T K A E K K G D E Y I I N G Q K M W I T N G G K A N W Y F L L A R S D P D P K A P A N K A F T G F I V E A D T P G I Q I G R K E L N M G Q R C S D T R G I V F E D V K V P K E N V L I G D G A G F K V A M G A F D K T R P V V A A G A V G L A Q R A L D E A T K Y A L E R K T F G K L L V E H Q A I S F M L A E M A M K V E L A R M S Y Q R A A W E V D S G R R N T Y Y A S I A K A F A G D I A N Q L A T D A V Q I L G G N G F N T E Y P V E K L M R D A K I Y Q I Y E G T S Q I Q R L I V A R E H I D K Y K N
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:2251268). Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175).
MCAD, Medium chain acyl-CoA dehydrogenase, MCADH, ACADM
Recombinant Human ACADM/MCAD protein is a Human Full Length protein, in the 26 to 421 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
>95% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, HPLC
No
No
Human
pH: 8.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.24% Tris
K A N R Q R E P G L G F S F E F T E Q Q K E F Q A T A R K F A R E E I I P V A A E Y D K T G E Y P V P L I R R A W E L G L M N T H I P E N C G G L G L G T F D A C L I S E E L A Y G C T G V Q T A I E G N S L G Q M P I I I A G N D Q Q K K K Y L G R M T E E P L M C A Y C V T E P G A G S D V A G I K T K A E K K G D E Y I I N G Q K M W I T N G G K A N W Y F L L A R S D P D P K A P A N K A F T G F I V E A D T P G I Q I G R K E L N M G Q R C S D T R G I V F E D V K V P K E N V L I G D G A G F K V A M G A F D K T R P V V A A G A V G L A Q R A L D E A T K Y A L E R K T F G K L L V E H Q A I S F M L A E M A M K V E L A R M S Y Q R A A W E V D S G R R N T Y Y A S I A K A F A G D I A N Q L A T D A V Q I L G G N G F N T E Y P V E K L M R D A K I Y Q I Y E G T S Q I Q R L I V A R E H I D K Y K N
Full Length
45.94 kDa
26 to 421
Recombinant
His tag N-Terminus
Liquid
The purity of ab173059 is greater than 95%, as determined by SEC-HPLC and reducing SDS-PAGE.
Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (PubMed:2251268). Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175).
Belongs to the acyl-CoA dehydrogenase family.
Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3.
Mitochondrion matrix
Dry Ice
-20°C
-20°C
Avoid freeze / thaw cycle
Previously labelled as ACADM.
This supplementary information is collated from multiple sources and compiled automatically.
ACADM also known as MCAD (medium-chain acyl-CoA dehydrogenase) is an enzyme that plays a significant role in fatty acid metabolism. It weighs approximately 44 kDa and expresses mainly in the liver heart and skeletal muscles. Functions of MCAD involve catalyzing the initial step in the mitochondrial fatty acid β-oxidation spiral specifically for the medium-chain fatty acids. This enzyme helps in the conversion of fatty acyl-CoA to trans-enoyl-CoA through dehydrogenation.
The enzyme facilitates energy production by breaking down medium-chain fatty acids within mitochondria. MCAD operates as a homotetramer complex where each subunit significantly contributes to its overall function. Such enzymatic activity is important for providing energy particularly when glycogen stores are low. Its efficient operation during fasting states indicates its importance in metabolic homeostasis.
Medium-chain acyl-CoA dehydrogenase (MCAD) is integral to the mitochondrial fatty acid beta-oxidation pathway. This pathway is a primary route for fatty acid catabolism ultimately leading to energy production in the form of ATP. MCAD's activity also relates to other fatty acid oxidation enzymes like VLCAD (very-long-chain acyl-CoA dehydrogenase) and SCAD (short-chain acyl-CoA dehydrogenase) which operate on different chain-length fatty acids cooperating to maintain energy balance.
MCAD deficiency is a common metabolic disorder that impairs the normal breakdown of fatty acids. This condition results in the accumulation of fatty acid intermediates leading to hypoketotic hypoglycemia during fasting periods. Individuals with MCAD deficiency may experience lethargy vomiting and seizures. The disorder connects with other enzymes such as LCHAD (long-chain 3-hydroxyacyl-CoA dehydrogenase) involved in similar pathways and deficiencies can result in related metabolic dysfunctions.
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