JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB95383

Recombinant Human ACADS/SCAD protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ACADS/SCAD protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

SCAD, Butyryl-CoA dehydrogenase, ACADS

1 Images
SDS-PAGE - Recombinant Human ACADS/SCAD protein (AB95383)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ACADS/SCAD protein (AB95383)

15% SDS-PAGE analysis of 3μg ab95383.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P16219

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Previously labelled as ACADS.
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKSISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMGFKIAMQTLDMGRIGIASQALGIAQTALDCAVNYAENRMAFGAPLTKLQVIQFKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P16219","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACADS protein also known as short-chain acyl-CoA dehydrogenase (SCAD) is an enzyme that plays a mechanical role in the mitochondrial fatty acid beta-oxidation process. It catalyzes the dehydrogenation of acyl-CoA substrates specifically short-chain fatty acids. The molecular mass of the ACADS protein is approximately 44 kDa. Expression of the ACADS protein occurs in the mitochondria of various tissues especially in liver and muscle tissues where fatty acid metabolism is active.
Biological function summary

The function of ACADS involves the conversion of fatty acids into acetyl-CoA an essential step in energy production. ACADS operates in concert with other enzymes in the mitochondrial matrix contributing to the beta-oxidation cycle. This enzyme is essential for maintaining energy homeostasis particularly during periods of fasting or increased energy demands. Although ACADS is not a component of a larger enzyme complex it works closely with other dehydrogenases that metabolize different chain lengths of fatty acids.

Pathways

The ACADS enzyme is critically involved in the fatty acid beta-oxidation pathway. This pathway is vital for energy production especially in cardiac and skeletal muscles. ACADS is functionally related to other enzymes such as MCAD (medium-chain acyl-CoA dehydrogenase) and VLCAD (very long-chain acyl-CoA dehydrogenase) through the sequential processing of fatty acids of varying chain lengths. Proper functioning of the beta-oxidation pathway ensures that fatty acids are systematically broken down to meet the body’s energetic needs.

Defects in ACADS are associated with metabolic disorders namely short-chain acyl-CoA dehydrogenase deficiency (SCADD). This condition can result in muscle weakness developmental delay and hypoglycemia. ACADS is also indirectly linked to disorders involving MCAD as both enzymes function within the same metabolic pathway. Accurate diagnosis and management of SCADD rely on understanding the role of ACADS and its interactions with interconnected proteins in the metabolic network.
style
left-column

Specifications

Form

Liquid

Additional notes

ab95383 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed : 11134486, PubMed : 21237683).

Sequence similarities

Belongs to the acyl-CoA dehydrogenase family.

Subcellular localisation

Mitochondrion matrix

style
left-column

Product protocols

style
left-column

Target data

Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains (PubMed : 11134486, PubMed : 21237683).
See full target information Short-chain specific acyl-CoA dehydrogenase, mitochondrial
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB156571

Anti-ACADS/SCAD antibody [EPR10862(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (AB156571)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com