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Recombinant Human ACE2 protein is a Human Fragment protein, in the 18 to 740 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

Biologically active

No

Sequence

Q S T I E E Q A K T F L D K F N H E A E D L F Y Q S S L A S W N Y N T N I T E E N V Q N M N N A G D K W S A F L K E Q S T L A Q M Y P L Q E I Q N L T V K L Q L Q A L Q Q N G S S V L S E D K S K R L N T I L N T M S T I Y S T G K V C N P D N P Q E C L L L E P G L N E I M A N S L D Y N E R L W A W E S W R S E V G K Q L R P L Y E E Y V V L K N E M A R A N H Y E D Y G D Y W R G D Y E V N G V D G Y D Y S R G Q L I E D V E H T F E E I K P L Y E H L H A Y V R A K L M N A Y P S Y I S P I G C L P A H L L G D M W G R F W T N L Y S L T V P F G Q K P N I D V T D A M V D Q A W D A Q R I F K E A E K F F V S V G L P N M T Q G F W E N S M L T D P G N V Q K A V C H P T A W D L G K G D F R I L M C T K V T M D D F L T A H H E M G H I Q Y D M A Y A A Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L S P D F Q E D N E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F K G E I P K D Q W M K K W W E M K R E I V G V V E P V P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T L Y Q F Q F Q E A L C Q A A K H E G P L H K C D I S N S T E A G Q K L F N M L R L G K S E P W T L A L E N V V G A K N M N V R P L L N Y F E P L F T W L K D Q N K N S F V G W S T D W S P Y A D Q S I K V R I S L K S A L G D K A Y E W N D N E M Y L F R S S V A Y A M R Q Y F L K V K N Q M I L F G E E D V R V A N L K P R I S F N F F V T A P K N V S D I I P R T E V E K A I R M S R S R I N D A F R L N D N S L E F L G I Q P T L G P P N Q P P V S V D H H H H H H

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant Human ACE2 protein is a Human Fragment protein, in the 18 to 740 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, HPLC

Predicted molecular weight

84.6 kDa

Amino acids

18 to 740

Sequence

Q S T I E E Q A K T F L D K F N H E A E D L F Y Q S S L A S W N Y N T N I T E E N V Q N M N N A G D K W S A F L K E Q S T L A Q M Y P L Q E I Q N L T V K L Q L Q A L Q Q N G S S V L S E D K S K R L N T I L N T M S T I Y S T G K V C N P D N P Q E C L L L E P G L N E I M A N S L D Y N E R L W A W E S W R S E V G K Q L R P L Y E E Y V V L K N E M A R A N H Y E D Y G D Y W R G D Y E V N G V D G Y D Y S R G Q L I E D V E H T F E E I K P L Y E H L H A Y V R A K L M N A Y P S Y I S P I G C L P A H L L G D M W G R F W T N L Y S L T V P F G Q K P N I D V T D A M V D Q A W D A Q R I F K E A E K F F V S V G L P N M T Q G F W E N S M L T D P G N V Q K A V C H P T A W D L G K G D F R I L M C T K V T M D D F L T A H H E M G H I Q Y D M A Y A A Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L S P D F Q E D N E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F K G E I P K D Q W M K K W W E M K R E I V G V V E P V P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T L Y Q F Q F Q E A L C Q A A K H E G P L H K C D I S N S T E A G Q K L F N M L R L G K S E P W T L A L E N V V G A K N M N V R P L L N Y F E P L F T W L K D Q N K N S F V G W S T D W S P Y A D Q S I K V R I S L K S A L G D K A Y E W N D N E M Y L F R S S V A Y A M R Q Y F L K V K N Q M I L F G E E D V R V A N L K P R I S F N F F V T A P K N V S D I I P R T E V E K A I R M S R S R I N D A F R L N D N S L E F L G I Q P T L G P P N Q P P V S V D H H H H H H

Accession
Q9BYF1-1
Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 7.4
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.001% Zinc chloride

Specifications

Form

Liquid

General info

Function

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).

Sequence similarities

Belongs to the peptidase M2 family.

Post-translational modifications

N-glycosylation on Asn-90 may limit SARS infectivity.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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