Recombinant Human ACE2 protein (Fc Chimera) is a Human Fragment protein, in the 18 to 615 aa range, expressed in HEK 293, with >95% purity, < 1 EU/mg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC.
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- Frontiers in immunology 13:9066872022Glucocorticoids Bind to SARS-CoV-2 S1 at Multiple Sites Causing Cooperative Inhibition of SARS-CoV-2 S1 Interaction With ACE2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHassan Sarker,Rashmi Panigrahi,Eugenio Hardy,J N Mark Glover,Shokrollah Elahi,Carlos Fernandez-PatronPubMed 35784352
- Small (Weinheim an der Bergstrasse, Germany) 18:e22008362022SARS-CoV-2 Spike Stem Protein Nanoparticles Elicited Broad ADCC and Robust Neutralization against Variants in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Ma,Ye Wang,Chunhong Dong,Gilbert X Gonzalez,Wandi Zhu,Joo Kim,Lai Wei,Sang-Moo Kang,Bao-Zhong WangPubMed 35607768
- Scientific reports 11:203832021Immunodominant linear B cell epitopes in the spike and membrane proteins of SARS-CoV-2 identified by immunoinformatics prediction and immunoassay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanokporn Polyiam,Waranyoo Phoolcharoen,Namphueng Butkhot,Chanya Srisaowakarn,Arunee Thitithanyanont,Prasert Auewarakul,Tawatchai Hoonsuwan,Marasri Ruengjitchatchawalya,Phenjun Mekvichitsaeng,Yaowaluck Maprang RoshormPubMed 34650130
- Cell reports. Medicine 1:1001422020Pharmacological Inhibition of Acid Sphingomyelinase Prevents Uptake of SARS-CoV-2 by Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Carpinteiro et. al.PubMed 33163980
- Scientific reports 10:176982020Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaewta Rattanapisit et. al.PubMed 33077899
- The Journal of biological chemistry :2020Sphingosine prevents binding of SARS-CoV-2 spike to its cellular receptor ACE2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael J Edwards et. al.PubMed 32917722
Key facts
- Purity
- >95%
- Endotoxin level
- < 1 EU/mg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, HPLC
- Biologically active
- No
Amino acid sequence
Q S T I E E Q A K T F L D K F N H E A E D L F Y Q S S L A S W N Y N T N I T E E N V Q N M N N A G D K W S A F L K E Q S T L A Q M Y P L Q E I Q N L T V K L Q L Q A L Q Q N G S S V L S E D K S K R L N T I L N T M S T I Y S T G K V C N P D N P Q E C L L L E P G L N E I M A N S L D Y N E R L W A W E S W R S E V G K Q L R P L Y E E Y V V L K N E M A R A N H Y E D Y G D Y W R G D Y E V N G V D G Y D Y S R G Q L I E D V E H T F E E I K P L Y E H L H A Y V R A K L M N A Y P S Y I S P I G C L P A H L L G D M W G R F W T N L Y S L T V P F G Q K P N I D V T D A M V D Q A W D A Q R I F K E A E K F F V S V G L P N M T Q G F W E N S M L T D P G N V Q K A V C H P T A W D L G K G D F R I L M C T K V T M D D F L T A H H E M G H I Q Y D M A Y A A Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L S P D F Q E D N E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F K G E I P K D Q W M K K W W E M K R E I V G V V E P V P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T L Y Q F Q F Q E A L C Q A A K H E G P L H K C D I S N S T E A G Q K L F N M L R L G K S E P W T L A L E N V V G A K N M N V R P L L N Y F E P L F T W L K D Q N K N S F V G W S T D W S P Y A D G G G G S G G G G S D K T H T C P P C P A P E A A G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2. Non-functional as a carboxypeptidase. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Alternative names
UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
Recommended products
Recombinant Human ACE2 protein (Fc Chimera) is a Human Fragment protein, in the 18 to 615 aa range, expressed in HEK 293, with >95% purity, < 1 EU/mg endotoxin level and suitable for SDS-PAGE, ELISA, HPLC.
Key facts
- Purity
- >95%
- Endotoxin level
- < 1 EU/mg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- SDS-PAGE, ELISA, HPLC
- Biologically active
- No
- Accession
- Q9BYF1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: PBS
Sequence info
Amino acid sequence
- Q S T I E E Q A K T F L D K F N H E A E D L F Y Q S S L A S W N Y N T N I T E E N V Q N M N N A G D K W S A F L K E Q S T L A Q M Y P L Q E I Q N L T V K L Q L Q A L Q Q N G S S V L S E D K S K R L N T I L N T M S T I Y S T G K V C N P D N P Q E C L L L E P G L N E I M A N S L D Y N E R L W A W E S W R S E V G K Q L R P L Y E E Y V V L K N E M A R A N H Y E D Y G D Y W R G D Y E V N G V D G Y D Y S R G Q L I E D V E H T F E E I K P L Y E H L H A Y V R A K L M N A Y P S Y I S P I G C L P A H L L G D M W G R F W T N L Y S L T V P F G Q K P N I D V T D A M V D Q A W D A Q R I F K E A E K F F V S V G L P N M T Q G F W E N S M L T D P G N V Q K A V C H P T A W D L G K G D F R I L M C T K V T M D D F L T A H H E M G H I Q Y D M A Y A A Q P F L L R N G A N E G F H E A V G E I M S L S A A T P K H L K S I G L L S P D F Q E D N E T E I N F L L K Q A L T I V G T L P F T Y M L E K W R W M V F K G E I P K D Q W M K K W W E M K R E I V G V V E P V P H D E T Y C D P A S L F H V S N D Y S F I R Y Y T R T L Y Q F Q F Q E A L C Q A A K H E G P L H K C D I S N S T E A G Q K L F N M L R L G K S E P W T L A L E N V V G A K N M N V R P L L N Y F E P L F T W L K D Q N K N S F V G W S T D W S P Y A D G G G G S G G G G S D K T H T C P P C P A P E A A G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K
- Accession
- Q9BYF1
- Protein length
- Fragment
- Predicted molecular weight
- 190.06 kDa
- Amino acids
- 18 to 615
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582).
- Sequence similarities
Belongs to the peptidase M2 family.
- Post-translational modifications
N-glycosylation on Asn-90 may limit SARS infectivity.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.
- Biological function summary
The ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.
- Pathways
ACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.
- Associated diseases and disorders
ACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.
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4 product images
ELISA - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
RBD Fc binding to immobilized ACE2 Fc fusion proteins. 96-well plates were coated with ab273687 and Recombinant Human ACE2 (mutated H374 N + H378 N) protein (Fc Chimera) (Recombinant Human ACE2 (mutated H374N + H378N) protein (Fc Chimera) ab273885) at 5 μg/ml. The SARS-CoV-2 RBD Fc fusion protein conjugated to HRP was titrated on a 3-fold serial dilution starting at 10 μg/ml.
ELISA - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
ab273687 binding to immobilized SARS-CoV-2 RBD Fc fusion protein. 96-well plate was coated with the SARS-CoV-2 RBD Fc fusion protein at 5 μg/ml. ab273687 was titrated on a 3-fold serial dilution starting at 10 μg/ml.
SDS-PAGE - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
SDS-PAGE analysis of 2.5 μg ab273687.
Lane 1: Non-reduced.
Lane 2: Reduced.
HPLC - Recombinant Human ACE2 protein (Fc Chimera) (ab273687)
HPLC analysis of ab273687.
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