Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active)
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Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.
View Alternative Names
ACC2, ACCB, ACACB, Acetyl-CoA carboxylase 2, ACC-beta
- FuncS
Unknown
Functional Studies - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (AB271358)
Specific activity of ab271358.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) (AB271358)
SDS-PAGE analysis of ab271358.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme controls fatty acid oxidation by regulating malonyl-CoA levels which in turn inhibits carnitine palmitoyltransferase 1 (CPT1) in the mitochondrial membrane. This action helps balance the partitioning between fatty acid synthesis and oxidation. ACCβ functions not in isolation but as part of a larger complex that can undergo polymerization or depolymerization impacting its activity. ACCβ impacts energy homeostasis by influencing cellular energy stores and metabolic flux.
Pathways
ACCβ plays a significant role in the fatty acid metabolism and insulin signaling pathways. It is a pivotal enzyme in the control of lipid biosynthesis and oxidation balance. Key proteins related to these pathways include AMP-activated protein kinase (AMPK) which phosphorylates and inactivates ACCβ therefore modulating its activity in response to energy levels. This regulation integrates nutritional signals with energy metabolism.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed : 16854592, PubMed : 19236960, PubMed : 19900410, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 19236960, PubMed : 20457939, PubMed : 20952656, PubMed : 26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).
Post-translational modifications
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.. Phosphorylation at Ser-222 by AMPK inactivates the enzyme (PubMed:12488245). Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity).
Subcellular localisation
Mitochondrion
Target data
Complementary Products
Product promise
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