JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202151

Recombinant Human Acid Phosphatase 2 protein (denatured)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Acid Phosphatase 2 protein (denatured) is a Human Fragment protein, in the 31 to 380 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Lysosomal acid phosphatase, LAP, ACP2

1 Images
SDS-PAGE - Recombinant Human Acid Phosphatase 2 protein (denatured) (AB202151)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Acid Phosphatase 2 protein (denatured) (AB202151)

15% SDS-PAGE analysis of ab202151 (3 μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P11117

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSRSLRFVTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVYDTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPYASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRLTEPVVPKDWQQECQLASGPADTE","proteinLength":"Fragment","predictedMolecularWeight":"42.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":31,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P11117","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid Phosphatase 2 also known as ACP2 or lysosomal acid phosphatase is an enzyme with a molecular mass of approximately 50 kDa. This enzyme locates mainly within lysosomes and operates in acidic conditions. It plays a mechanical role by hydrolyzing phosphate esters into inorganic phosphate and alcohol making it an important actor in the breakdown of biomolecules. ACP2 appears most abundantly in tissues such as the liver spleen and other organs involved in high metabolic activities.
Biological function summary

Acid Phosphatase 2 participates in the degradation of intracellular biomolecules and recycling of phosphate groups. It works in association with other lysosomal enzymes forming an important component of the lysosomal degradation complex. Within the lysosome it assists in processing macromolecules like nucleotides lipids and glycans contributing to cellular nutrient and energy homeostasis. By cleaving phosphate from a diverse range of substrates ACP2 aids in maintaining cellular function and growth.

Pathways

Acid phosphatases influence the lysosomal degradation pathway and play roles in phosphate metabolism. ACP2 interacts with enzymes involved in lysosomal functions like cathepsins and glucocerebrosidase which drive the breakdown of cellular debris. Furthermore ACP2 activity is intertwined with the mTOR signaling pathway impacting cellular growth and autophagy processes by regulating nutrient availability at the cellular level. This integration into metabolic pathways highlights its significance in regulating metabolic flux between degradation and biosynthesis.

Acid phosphatase 2 holds relevance to lysosomal storage disorders and several types of cancer. Mutations or deficiencies in ACP2 lead to issues such as lysosomal storage diseases where accumulated substrates disrupt normal cellular function. Moreover altered ACP2 activity correlates with prostate cancer development and progression where it connects with proteins like prostate-specific antigen (PSA) influencing tumor behaviors. These associations underline the importance of ACP2 in maintaining cellular health and its role when dysregulated in disease states.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Sequence similarities

Belongs to the histidine acid phosphatase family.

Post-translational modifications

The membrane-bound form is converted to the soluble form by sequential proteolytic processing. First, the C-terminal cytoplasmic tail is removed. Cleavage by a lysosomal protease releases the soluble form in the lysosome lumen.. N-glycosylated. The intermediates formed during enzymatic deglycosylation suggest that all eight predicted N-glycosylation sites are used.

Subcellular localisation

Lysosome membrane

style
left-column

Product protocols

style
left-column

Target data

See full target information ACP2
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB83367

Acid Phosphatase Assay Kit (Colorimetric)

Functional Studies - Acid Phosphatase Assay Kit (Colorimetric) (AB83367)

  • 0
  • 0
Proteins & Peptides

AB165455

Recombinant Human ZNF358 protein

SDS-PAGE - Recombinant Human ZNF358 protein (AB165455)

  • 0
  • 0
Proteins & Peptides

AB164919

Recombinant Human Zinc finger protein 276

SDS-PAGE - Recombinant Human Zinc finger protein 276 (AB164919)

  • 0
  • 0
Proteins & Peptides

AB166020

Recombinant Human YTHDF3 protein

SDS-PAGE - Recombinant Human YTHDF3 protein (AB166020)

  • 0
  • 0
Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com