Recombinant human Acid phosphatase/ACP1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A E Q A T K S V L F V C L G N I C R S P I A E A V F R K L V T D Q N I S E N W V I D S G A V S D W N V G R S P D P R A V S C L R N H G I H T A H K A R Q I T K E D F A T F D Y I L C M D E S N L R D L N R K S N Q V K T C K A K I E L L G S Y D P Q K Q L I I E D P Y Y G N D S D F E T V Y Q Q C V R C C R A F L E K A H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2. Isoform 3. Does not possess phosphatase activity.
Alternative names
Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1
Recommended products
Recombinant human Acid phosphatase/ACP1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: > 60,000 unit/mg of protein. Unit definition: One unit is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Accession
- P24666-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 6
Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.0584% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A E Q A T K S V L F V C L G N I C R S P I A E A V F R K L V T D Q N I S E N W V I D S G A V S D W N V G R S P D P R A V S C L R N H G I H T A H K A R Q I T K E D F A T F D Y I L C M D E S N L R D L N R K S N Q V K T C K A K I E L L G S Y D P Q K Q L I I E D P Y Y G N D S D F E T V Y Q Q C V R C C R A F L E K A H
- Accession
- P24666
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab87493 is purified using conventional chromatography techniques.
General info
- Function
Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.
- Sequence similarities
Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
- Post-translational modifications
Isoform 2
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method). This product was previously labelled as Acid Phosphatase
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
- Biological function summary
The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.
- Pathways
The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.
- Associated diseases and disorders
Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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1 product image
SDS-PAGE - Recombinant human Acid phosphatase/ACP1 protein (ab87493)
15% SDS-PAGE showing 3μg ab87493 at approximately 20.1kDa.
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