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AB87493

Recombinant human Acid phosphatase/ACP1 protein (His tag N-Terminus)

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Recombinant human Acid phosphatase/ACP1 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1

1 Images
SDS-PAGE - Recombinant human Acid phosphatase/ACP1 protein (His tag N-Terminus) (AB87493)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Acid phosphatase/ACP1 protein (His tag N-Terminus) (AB87493)

15% SDS-PAGE showing 3μg ab87493 at approximately 20.1kDa.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity: > 60,000 unit/mg of protein. Unit definition: One unit is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Accession

P24666

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 6 Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.0584% EDTA, 0.00174% PMSF

storage-buffer

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Complementary Products

Proteins & Peptides

AB51277

Recombinant human PTP1B protein (Active) (Tag Free)

SDS-PAGE - Recombinant human PTP1B protein (Active) (Tag Free) (AB51277)

  • 0
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Primary Antibodies

AB235448

Anti-Acid phosphatase antibody [EPR21787]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21787] (AB235448)

  • 0
  • 0
Primary Antibodies

AB68474

Anti-eIF4B antibody [EP2299Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Endotoxin level: < 1.0 EU per 1 μg of protein (determined by LAL method). This product was previously labelled as Acid Phosphatase
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAEQATKSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWVIDSGAVSDWNVGRSPDPRAVSCLRNHGIHTAHKARQITKEDFATFDYILCMDESNLRDLNRKSNQVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFLEKAH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P24666","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as ACP1 or protein tyrosine phosphatase is an enzyme with a mass of approximately 18 kDa. It works by removing phosphate groups from tyrosine residues on proteins which modulates protein function. This phosphatase is expressed in various tissues including the pancreas and liver and is present in immune cells. In scientific studies ACP1 can also be studied in HEK 293 cells a commonly used human cell line for in vitro assays.
Biological function summary

The acid phosphatase enzyme plays an important role in cellular signaling and regulation of metabolic processes. It belongs to the enzyme family of protein tyrosine phosphatases which are key in dephosphorylating proteins and regulating signal transduction pathways. ACP1 does not function as part of a large protein complex but it still affects cellular processes by interacting with multiple molecular partners.

Pathways

The acid phosphatase enzyme is involved in key cellular signaling pathways including the insulin signaling pathway and T-cell receptor signaling. These pathways influence glucose metabolism and immune response. In these pathways ACP1 interacts with proteins such as the insulin receptor substrate and Lck influencing phosphorylation states and cellular activities.

Acid phosphatase has connections to conditions like autoimmune diseases and type 2 diabetes. In autoimmune disorders altered ACP1 activity can affect immune cell signaling. Studies link increased enzyme activity or genetic variations to these conditions impacting associated proteins like the insulin receptor in diabetes or TCR complex proteins in immune dysfunctions providing insight into how aberrations in ACP1 can contribute to disease pathogenesis.
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Specifications

Form

Liquid

Additional notes

ab87493 is purified using conventional chromatography techniques.

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General info

Function

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.. Isoform 3. Does not possess phosphatase activity.

Sequence similarities

Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Post-translational modifications

Isoform 2. Phosphorylated by LCK (PubMed:10336608, PubMed:9038134). Phosphorylation at Tyr-132 increases its phosphatase activity (PubMed:9038134).. Isoform 3. Not phosphorylated.

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Product protocols

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Target data

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.. Isoform 3. Does not possess phosphatase activity.
See full target information ACP1
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