Recombinant Human Aconitase 2 protein is a Human Full Length protein, in the 1 to 780 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A P Y S L L V T R L Q K A L G V R Q Y H V A S V L C Q R A K V A M S H F E P N E Y I H Y D L L E K N I N I V R K R L N R P L T L S E K I V Y G H L D D P A S Q E I E R G K S Y L R L R P D R V A M Q D A T A Q M A M L Q F I S S G L S K V A V P S T I H C D H L I E A Q V G G E K D L R R A K D I N Q E V Y N F L A T A G A K Y G V G F W K P G S G I I H Q I I L E N Y A Y P G V L L I G T D S H T P N G G G L G G I C I G V G G A D A V D V M A G I P W E L K C P K V I G V K L T G S L S G W S S P K D V I L K V A G I L T V K G G T G A I V E Y H G P G V D S I S C T G M A T I C N M G A E I G A T T S V F P Y N H R M K K Y L S K T G R E D I A N L A D E F K D H L V P D P G C H Y D Q L I E I N L S E L K P H I N G P F T P D L A H P V A E V G K V A E K E G W P L D I R V G L I G S C T N S S Y E D M G R S A A V A K Q A L A H G L K C K S Q F T I T P G S E Q I R A T I E R D G Y A Q I L R D L G G I V L M A P Y S L L V T R L Q K A L G V R Q Y H V A S V L C Q R A K V A M S H F E P N E Y I H Y D L L E K N I N I V R K R L N R P L T L S E K I V Y G H L D D P A S Q E I E R G K S Y L R L R P D R V A M Q D A T A Q M A M L Q F I S S G L S K V A V P S T I H C D H L I E A Q V G G E K D L R R A K D I N Q E V Y N F L A T A G A K Y G V G F W K P G S G I I H Q I I L E N Y A Y P G V L L I G T D S H T P N G G G L G G I C I G V G G A D A V D V M A G I P W E L K C P K V I G V K L T G S L S G W S S P K D V I L K V A G I L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Alternative names
Aconitase, Citrate hydro-lyase, ACO2
Recommended products
Recombinant Human Aconitase 2 protein is a Human Full Length protein, in the 1 to 780 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q99798-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M A P Y S L L V T R L Q K A L G V R Q Y H V A S V L C Q R A K V A M S H F E P N E Y I H Y D L L E K N I N I V R K R L N R P L T L S E K I V Y G H L D D P A S Q E I E R G K S Y L R L R P D R V A M Q D A T A Q M A M L Q F I S S G L S K V A V P S T I H C D H L I E A Q V G G E K D L R R A K D I N Q E V Y N F L A T A G A K Y G V G F W K P G S G I I H Q I I L E N Y A Y P G V L L I G T D S H T P N G G G L G G I C I G V G G A D A V D V M A G I P W E L K C P K V I G V K L T G S L S G W S S P K D V I L K V A G I L T V K G G T G A I V E Y H G P G V D S I S C T G M A T I C N M G A E I G A T T S V F P Y N H R M K K Y L S K T G R E D I A N L A D E F K D H L V P D P G C H Y D Q L I E I N L S E L K P H I N G P F T P D L A H P V A E V G K V A E K E G W P L D I R V G L I G S C T N S S Y E D M G R S A A V A K Q A L A H G L K C K S Q F T I T P G S E Q I R A T I E R D G Y A Q I L R D L G G I V L M A P Y S L L V T R L Q K A L G V R Q Y H V A S V L C Q R A K V A M S H F E P N E Y I H Y D L L E K N I N I V R K R L N R P L T L S E K I V Y G H L D D P A S Q E I E R G K S Y L R L R P D R V A M Q D A T A Q M A M L Q F I S S G L S K V A V P S T I H C D H L I E A Q V G G E K D L R R A K D I N Q E V Y N F L A T A G A K Y G V G F W K P G S G I I H Q I I L E N Y A Y P G V L L I G T D S H T P N G G G L G G I C I G V G G A D A V D V M A G I P W E L K C P K V I G V K L T G S L S G W S S P K D V I L K V A G I L
- Accession
- Q99798
- Protein length
- Full Length
- Predicted molecular weight
- 111.2 kDa
- Amino acids
- 1 to 780
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
- Sequence similarities
Belongs to the aconitase/IPM isomerase family.
- Post-translational modifications
Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aconitase 2 also known as ACO2 is an enzyme involved in the citric acid cycle which plays an important role in energy production within the cell. This enzyme converts citrate to isocitrate via a cis-aconitate intermediate an essential step in the cycle that generates ATP. Aconitase 2 has a molecular mass of approximately 83 kDa and is primarily expressed in the mitochondria of eukaryotic cells where it functions optimally due to the organelle's environment.
- Biological function summary
Aconitase 2 supports cellular energy metabolism by facilitating the conversion of citrate derivatives. It is an iron-sulfur protein integral to both the cytosolic aconitase and mitochondrial varieties that functions as part of the mitochondrial aconitase complex. The enzyme's iron-sulfur cluster gets oxidized under different conditions causing the enzyme to act as a regulatory factor in response to iron levels further linking it with cellular iron metabolism.
- Pathways
Aconitase 2 plays a significant role in the tricarboxylic acid (TCA) cycle commonly known as the Krebs cycle and the iron regulatory pathway. In the TCA cycle aconitase 2 acts after citrate synthase and before isocitrate dehydrogenase establishing its role in the middle of the cycle. It is functionally related to iron regulatory proteins particularly IRP1 which can interchangeably bind to iron-responsive elements or possess aconitase activity in its Fe-containing form.
- Associated diseases and disorders
Disruptions in aconitase 2 activity are linked to conditions like Friedreich's ataxia and cancer. Friedreich's ataxia involves mitochondrial dysfunction wherein the decreased activity of aconitase 2 leads to impaired energy metabolism contributing to neurodegeneration. In cancers altered aconitase 2 function can impact cellular proliferation by modifying energy metabolism pathways. Aconitase 2 also interacts with the protein frataxin in the context of these conditions underlining its involvement in disease mechanisms related to mitochondrial dysfunction and iron metabolism.
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1 product image
SDS-PAGE - Recombinant Human Aconitase 2 protein (ab114378)
ab114378 on 12.5% SDS-PAGE stained with Coomassie Blue.
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