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AB157832

Recombinant Human Acrosin protein (GST tag N-Terminus)

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Recombinant Human Acrosin protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 421 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ACRS, ACR, Acrosin

1 Images
SDS-PAGE - Recombinant Human Acrosin protein (GST tag N-Terminus) (AB157832)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Acrosin protein (GST tag N-Terminus) (AB157832)

ab157832 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P10323

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

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AB159227

Recombinant Human Protamine 2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Protamine 2 protein (AB159227)

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Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
  • 6
Primary Antibodies

AB203289

Anti-Acrosin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acrosin antibody (AB203289)

  • 0
  • 0
Proteins & Peptides

AB276578

Recombinant Human Statherin protein (Fc Chimera)

SDS-PAGE - Recombinant Human Statherin protein (Fc Chimera) (AB276578)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MVEMLPTAILLVLAVSVVAKDNATCDGPCGLRFRQNPQGGVRIVGGKAAQHGAWPWMVSLQIFTYNSHRYHTCGGSLLNSRWVLTAAHCFVGKNNVHDWRLVFGAKEITYGNNKPVKAPLQERYVEKIIIHEKYNSATEGNDIALVEITPPISCGRFIGPGCLPHFKAGLPRGSQSCWVAGWGYIEEKAPRPSSILMEARVDLIDLDLCNSTQWYNGRVQPTNVCAGYPVGKIDTCQGDSGGPLMCKDSKESAYVVVGITSWGVGCARAKRPGIYTATWPYLNWIASKIGSNALRMIQSATPPPPTTRPPPIRPPFSHPISAHLPWYFQPPPRPLPPRPPAAQPRPPPSPPPPPPPPASPLPPPPPPPPPTPSSTTKLPQGLSFAKRLQQLIEVLKGKTYSDGKNHYDMETTELPELTSTS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":421,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P10323","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acrosin also known as ZPC and with a mass of approximately 40 kDa acts as a proteolytic enzyme in sperm cells. It falls under the category of serine proteases. Acrosin is synthesized in the acrosome of spermatozoa and gets activated during the sperm's travel through the female reproductive tract. The enzyme's main mechanical function is to digest the zona pellucida the outer glycoprotein layer of the egg aiding in sperm penetration and fertilization process.
Biological function summary

Enzymes like acrosin play significant roles in fertilization. Acrosin remains stored as proacrosin its inactive zymogen form within the acrosome until it is activated. This protein operates as part of a complex network that includes other acrosomal proteins. The activation of acrosin is tightly controlled ensuring the precise timing and localization of its enzymatic activity required for efficient sperm-egg fusion.

Pathways

Sperm motility and capacitation pathways integrate acrosin activity. These pathways are pivotal during fertilization as they govern the sperm's ability to reach and penetrate the egg. Acrosin interacts with enzymes like hyaluronidase in pathways where the digestion of the zona pellucida is necessary. This interaction ensures that sperm cells effectively breach the barriers surrounding the oocyte for successful fertilization.

Fertilization problems can connect with acrosin function anomalies. Improper activation or deficits in acrosin activity may contribute to infertility. Additionally anomalies in related proteins such as other serine proteases or those within the sperm's acrosome can lead to unsuccessful sperm-egg interactions. Research focuses on exploring these relationships to identify potential diagnostic markers or therapeutic targets for addressing infertility issues associated with acrosomal proteins like acrosin.
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Specifications

Form

Liquid

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General info

Function

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Sequence similarities

Belongs to the peptidase S1 family.

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Product protocols

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Target data

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
See full target information ACR
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