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AB239557

Recombinant Human ACSS1 protein (His tag)

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Recombinant Human ACSS1 protein (His tag) is a Human Full Length protein, in the 38 to 689 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ACAS2L, KIAA1846, ACSS1, Acetate--CoA ligase 2, Acetyl-CoA synthetase 2, Acyl-CoA synthetase short-chain family member 1, Propionate--CoA ligase, AceCS2

1 Images
SDS-PAGE - Recombinant Human ACSS1 protein (His tag) (AB239557)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ACSS1 protein (His tag) (AB239557)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239557.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9NUB1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK","proteinLength":"Full Length","predictedMolecularWeight":"75.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":689,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NUB1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACSS1 protein also known as Acyl-CoA Synthetase Short Chain Family Member 1 plays an important role in converting acetate into acetyl-CoA an important molecule for various metabolic processes. This conversion occurs in mitochondria where ACSS1 predominantly operates. The protein has a mass of approximately 72 kDa. Expression patterns of ACSS1 indicate high levels in heart and skeletal muscle tissues highlighting its importance in energy-demanding cells.
Biological function summary

ACSS1 contributes to cellular energy homeostasis by facilitating acetate uptake and utilization. It functions as part of a metabolic network rather than a static complex. Acetyl-CoA produced by ACSS1 is important for energy production as well as lipid synthesis and histone acetylation which is important for regulating gene expression. This versatility makes ACSS1 a significant player in metabolic flexibility.

Pathways

ACSS1 features prominently in the acetate metabolism pathway coupling with the tricarboxylic acid (TCA) cycle to support ATP production. The protein influences lipid synthesis and cellular processes through its interactions with other proteins like ACSS2 and ACLY which are involved in related metabolic pathways. By linking acetate metabolism with broader biochemical functions ACSS1 helps maintain a balance in mitochondrial energy supplies.

ACSS1 has exhibited links with metabolic disturbances such as cardiomyopathy and non-alcoholic fatty liver disease (NAFLD). Disturbed acetate metabolism can disrupt heart muscle functionality and energy supply connecting ACSS1 with these conditions. The protein also associates with SIRT3 an important regulator in mitochondrial health indicating its potential role in these metabolic-related diseases. Understanding these connections can provide insights into ACSS1's therapeutic potential in metabolic disorders.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed : 16788062). Acetate is the preferred substrate (PubMed : 16788062). Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Post-translational modifications

Reversibly acetylated on Lys-642 (PubMed:16788062). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed : 16788062). Acetate is the preferred substrate (PubMed : 16788062). Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).
See full target information ACSS1
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