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AB157842

Recombinant Human ACTG2 protein (GST tag N-Terminus)

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Recombinant Human ACTG2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 376 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

ACTA3, ACTL3, ACTSG, ACTG2, Alpha-actin-3, Gamma-2-actin, Smooth muscle gamma-actin

1 Images
SDS-PAGE - Recombinant Human ACTG2 protein (GST tag N-Terminus) (AB157842)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ACTG2 protein (GST tag N-Terminus) (AB157842)

ab157842 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P63267

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB156302

Anti-muscle Actin antibody [EPR8484] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR8484] - Loading Control (AB156302)

  • 2
  • 1
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Proteins & Peptides

AB112326

Recombinant Human beta-cardiac myosin heavy chain protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human beta-cardiac myosin heavy chain protein (GST tag N-Terminus) (AB112326)

  • 0
  • 0
Proteins & Peptides

AB310318

Recombinant HIV1 Gag protein (His-tag)

Mass Spectrometry - Recombinant HIV1 Gag protein (His-tag) (AB310318)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Protein concentration is above or equal to 0.05 mg/ml.
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Sequence info

[{"sequence":"MCEEETTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKPEYDEAGPSIVHRKCF","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":376,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P63267","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ACTG2 protein also known as gamma-2 actin plays a role in the mechanical structure and function of muscle tissues. It is a part of the actin family of proteins which are essential components of the cytoskeleton. ACTG2 has a molecular mass of approximately 42 kDa. This protein is expressed mainly in smooth muscles where it contributes to the structural integrity and contractile function by forming actin filaments.
Biological function summary

The ACTG2 protein is critical in maintaining and regulating the contractility of smooth muscle tissues. It does so by being part of the thin filament complex that interacts with myosin for muscle contraction. This complex is essential for the normal functioning of various organs such as blood vessels gastrointestinal tract and respiratory tract which require smooth muscle contraction for their operation.

Pathways

ACTG2 is integral to the contractile mechanism pathways of smooth muscle cells. It participates in the actin-myosin interaction that converts chemical energy into mechanical work a process essential for muscle contraction in smooth muscle tissue. This actin protein relates to other contractile proteins like myosin heavy chain and tropomyosin which are important within these pathways for effective muscle function.

ACTG2 mutations have connections to visceral myopathy and other types of smooth muscle dysfunctions. Visceral myopathy affects the smooth muscle tissues of the gastrointestinal tract leading to issues like chronic intestinal pseudo-obstruction. In this context ACTG2 mutations can affect interactions with proteins like myosin disrupting contractility and resulting in such disorders.
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Specifications

Form

Liquid

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General info

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Sequence similarities

Belongs to the actin family.

Post-translational modifications

Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.. Actin, gamma-enteric smooth muscle, intermediate form. N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP.. Monomethylation at Lys-85 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.. Methylated at His-74 by SETD3.. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-51 of one monomer and Glu-271 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
See full target information ACTG2
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Product promise

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