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Recombinant Human Activin A (Active) protein is a Human Fragment protein, in the 311 to 426 aa range, expressed in HEK 293, with >95% purity and < 1 EU/µg endotoxin level.

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Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Biologically active
Yes

Amino acid sequence

G L E C D G K V N I C C K K Q F F V S F K D I G W N D W I I A P S G Y H A N Y C E G E C P S H I A G T S G S S L S F H S T V I N H Y R M R G H S P F A N L K S C C V P T K L R P M S M L Y Y D D G Q N I I K K D I Q N M I V E E C G C S

Target data

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Alternative names

Recommended products

Recombinant Human Activin A (Active) protein is a Human Fragment protein, in the 311 to 426 aa range, expressed in HEK 293, with >95% purity and < 1 EU/µg endotoxin level.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
HEK 293 cells
Biological activity
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL.
Accession
P08476-1
Animal free
No
Species
Human
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Concentration
Loading...
Storage buffer

Constituents: 91.99% PBS, 8% Trehalose

Sequence info

Amino acid sequence

G L E C D G K V N I C C K K Q F F V S F K D I G W N D W I I A P S G Y H A N Y C E G E C P S H I A G T S G S S L S F H S T V I N H Y R M R G H S P F A N L K S C C V P T K L R P M S M L Y Y D D G Q N I I K K D I Q N M I V E E C G C S
Accession
P08476
Protein length
Fragment
Predicted molecular weight
12.97 kDa
Amino acids
311 to 426
Nature
Recombinant

Specifications

Form
Lyophilized

General info

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Sequence similarities

Belongs to the TGF-beta family.

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage duration
A few days
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C, -80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Activin A also known by its gene name INHBA is a protein involved in various mechanical processes in the human body. This protein forms part of the transforming growth factor-beta (TGF-β) superfamily and has a molecular weight of approximately 25 kDa for its dimeric forms. Activin A is expressed in many tissues including the pituitary gland reproductive organs and placenta. Researchers often use techniques like the Activin A ELISA test kit to measure its concentration in biological samples.

Biological function summary

Activin A plays a significant role in regulating cell differentiation proliferation and apoptosis. As part of a complex Activin A interacts with specific receptors on cell surfaces initiating intracellular signaling cascades that influence these vital processes. It acts as a growth factor in the reproductive system influencing follicle-stimulating hormone (FSH) secretion and is important in embryonic development and wound healing.

Pathways

Activin A participates in pathways such as the TGF-β signaling pathway and the SMAD protein signaling pathway. Within these pathways it interacts with receptor proteins like type I and type II Activin receptors and downstream SMAD proteins. These interactions enable Activin A to regulate gene expression relevant to various cellular functions.

Associated diseases and disorders

Altered Activin A levels have associations with conditions like cancer and fibrotic diseases. Its overexpression links to some cancer types including colorectal and prostate cancer where it influences tumor growth and metastasis. Moreover its interaction with proteins like myostatin may contribute to muscle wasting disorders. The dysregulation of these pathways highlights the significance of Activin A in pathophysiology.

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