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AB271359

Recombinant Human Activin Receptor Type IA protein (Active) (Biotin)

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Recombinant Human Activin Receptor Type IA protein (Active) (Biotin) is a Human Fragment protein, in the 147 to 509 aa range, expressed in Baculovirus infected Sf9 cells, with 90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

ACVRLK2, ACVR1, Activin receptor type-1, Activin receptor type I, Activin receptor-like kinase 2, Serine/threonine-protein kinase receptor R1, TGF-B superfamily receptor type I, ACTR-I, ALK-2, SKR1, TSR-I

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Functional Studies - Recombinant Human Activin Receptor Type IA protein (Active) (Biotin) (AB271359)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human Activin Receptor Type IA protein (Active) (Biotin) (AB271359)

Kinase activity of ab271359 using casein as substrate.

SDS-PAGE - Recombinant Human Activin Receptor Type IA protein (Active) (Biotin) (AB271359)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Activin Receptor Type IA protein (Active) (Biotin) (AB271359)

SDS-PAGE analysis of ab271359.

Key facts

Purity

90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

DDDDK tag N-Terminus Avi tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Kinase assay using casein as substrate.

Enzyme reactions include 40 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA, 10 μM ATP, 0.2 mg/mL casein, 3 mM DTT, and various amounts of ab271359—incubated 45 minutes at 30 °C. Followed by addition of 25 μL of luminescent reagent—incubated 45 minutes at room temperature. Followed by addition of 50 μl of proprietary detection reagent —incubated 45 minutes at room temperature. Luminescence was measured.

Conjugation

Biotin

Excitation/Emission
Accession

Q04771

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Enzymatically biotin-labeled using Avi-tag™ technology
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"45 kDa","actualMolecularWeight":null,"aminoAcidEnd":509,"aminoAcidStart":147,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q04771","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"Avi","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activin Receptor Type IA also known as ACVR1 or ALK2 is a type I receptor serine/threonine kinase. It has a mass of approximately 55 kDa. Activin Receptor Type IA is widely expressed in various tissues including skeletal muscle heart and central nervous system. It functions through the binding of ligands like activins and bone morphogenetic proteins (BMPs) which triggers intracellular signaling cascades important for cellular processes.
Biological function summary

Activin Receptor Type IA plays a significant role in several cellular activities including growth and differentiation. It operates as part of a receptor complex with type II receptors like ACVR2A protein which facilitates ligand binding and phosphorylation. Activin receptor is essential for the regulation of mesoderm induction and organ development notably in the skeletal and muscular systems. Its interaction with activin protein family members influences various developmental processes.

Pathways

Activin Receptor Type IA is an integral component of the TGF-beta signaling pathway and BMP pathway. These pathways are vital for embryonic development and tissue homeostasis. Activin receptor interacts with the SMAD family of proteins which mediate signal transduction to the nucleus influencing gene expression. Through these pathways it relates functionally to SMAD1 and SMAD2 proteins that contribute to cell proliferation and differentiation dynamics.

Activin Receptor Type IA is associated with fibrodysplasia ossificans progressiva (FOP) and cancer. Mutations in the ACVR1 gene can lead to abnormal bone growth seen in FOP commonly due to erroneous activation of the BMP signaling pathway. In some cancers the dysregulation of activin receptor signaling can alter cell proliferation rates placing it in connection with proteins involved in oncogenic processes. Targeting activin receptor pathways might offer therapeutic potential in certain diseases by modulating or restoring its normal signaling capability.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Bone morphogenetic protein (BMP) type I receptor that is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation (PubMed : 20628059, PubMed : 22977237). As a type I receptor, forms heterotetrameric receptor complexes with the type II receptors AMHR2, ACVR2A or ACVR2B (PubMed : 17911401). Upon binding of ligands such as BMP7 or GDF2/BMP9 to the heteromeric complexes, type II receptors transphosphorylate ACVR1 intracellular domain (PubMed : 25354296). In turn, ACVR1 kinase domain is activated and subsequently phosphorylates SMAD1/5/8 proteins that transduce the signal (PubMed : 9748228). In addition to its role in mediating BMP pathway-specific signaling, suppresses TGFbeta/activin pathway signaling by interfering with the binding of activin to its type II receptor (PubMed : 17911401). Besides canonical SMAD signaling, can activate non-canonical pathways such as p38 mitogen-activated protein kinases/MAPKs (By similarity). May promote the expression of HAMP, potentially via its interaction with BMP6 (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

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Product protocols

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Target data

Bone morphogenetic protein (BMP) type I receptor that is involved in a wide variety of biological processes, including bone, heart, cartilage, nervous, and reproductive system development and regulation (PubMed : 20628059, PubMed : 22977237). As a type I receptor, forms heterotetrameric receptor complexes with the type II receptors AMHR2, ACVR2A or ACVR2B (PubMed : 17911401). Upon binding of ligands such as BMP7 or GDF2/BMP9 to the heteromeric complexes, type II receptors transphosphorylate ACVR1 intracellular domain (PubMed : 25354296). In turn, ACVR1 kinase domain is activated and subsequently phosphorylates SMAD1/5/8 proteins that transduce the signal (PubMed : 9748228). In addition to its role in mediating BMP pathway-specific signaling, suppresses TGFbeta/activin pathway signaling by interfering with the binding of activin to its type II receptor (PubMed : 17911401). Besides canonical SMAD signaling, can activate non-canonical pathways such as p38 mitogen-activated protein kinases/MAPKs (By similarity). May promote the expression of HAMP, potentially via its interaction with BMP6 (By similarity).
See full target information ACVR1
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