JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB134842

Recombinant Human ACTL6A protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ACTL6A protein is a Human Full Length protein, in the 2 to 429 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

BAF53, BAF53A, INO80K, ACTL6A, Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, INO80 complex subunit K

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O96019

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB131272

Anti-ACTL6A antibody [EPR7443]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACTL6A antibody [EPR7443] (AB131272)

  • 0
  • 0
Proteins & Peptides

AB82237

Recombinant human BRG1 protein

Western blot - Recombinant human BRG1 protein (AB82237)

  • 0
  • 0
Primary Antibodies

AB17968

Anti-BRD8/p120 antibody

Western blot - Anti-BRD8/p120 antibody (AB17968)

  • 0
  • 0
Primary Antibodies

AB109379

Anti-RCC1 antibody [EPR5857]

RabMAb|Recombinant

Western blot - Anti-RCC1 antibody [EPR5857] (AB109379)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MASMTGGQENGRGEFSGGVYGGDEVGALVFDIGSYTVRAGYAGEDCPKVDFPTAIGMVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVKSEASLHPVLMSEAPWNTRAKREKLTELMFEHYNIPAFFLCKTAVLTAFANGRSTGLILDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFITMQCRELFQEMNIELVPPYMIASKEAVREGSPANWKRKEKLPQVTRSWHNYMCNCVIQDFQASVLQVSDSTYDEQVAAQMPTVHYEFPNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTMLGVSHVVTTSVGMCDIDIRPGLYGSVIVAGGNTLIQSFTDRLNRELSQKTPPSMRLKLIANNTTVERRFSSWIGGSILASLGTFQQMWISKQEYEEGGKQCVERKCP","proteinLength":"Full Length","predictedMolecularWeight":"48.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":429,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O96019","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTL6A also known as BAF53A or Arp4 is a member of the actin-related protein family with a molecular mass of approximately 53 kDa. This protein plays an important role in chromatin remodeling facilitating transcriptional regulation by altering nucleosome positioning. ACTL6A shows expression in various tissues with high levels found in neurons and certain cancer cell types. Its function depends on local cellular context which influences its involvement in specific gene expression profiles.
Biological function summary

ACTL6A supports transcriptional regulation by integrating into the SWI/SNF chromatin-remodeling complex. This complex which includes other key proteins coordinates to reposition nucleosomes aiding transcription factors in accessing target DNA sequences. ACTL6A contributes to regulation of gene expression patterns that underline cell identity and differentiation reflecting its critical role in neuronal and cancer cell biology.

Pathways

The SWI/SNF complex which includes ACTL6A integrates into pathways that control cell proliferation and differentiation. Specifically the Wnt signaling pathway uses ACTL6A to regulate gene expression necessary for proper cell function. Proteins like BRG1 and BRM serve as partners in this process helping to execute transcriptional activation. By participating in these pathways ACTL6A emphasizes its role in conserving cellular homeostasis.

ACTL6A exhibits associations with oncogenesis and neural disorders. Researchers observed its link to the development of glioblastoma where it influences tumor progression through interactions with other SWI/SNF members like SMARCA4. Additionally abnormalities in ACTL6A function relate to neurodevelopmental disorders emphasizing its importance in neuronal growth and differentiation. Its connection to these diseases highlights the potential of ACTL6A as a target for therapeutic interventions.
style
left-column

Specifications

Form

Liquid

Additional notes

ab134842 was expressed in E. coli as inclusion bodies, refolded using a temperature shift inclusion body refolding technology and chromatographically purified.

style
left-column

General info

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Sequence similarities

Belongs to the actin family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
See full target information ACTL6A
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com