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AB172851

Recombinant Human ACTR8 protein

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Recombinant Human ACTR8 protein is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

ARP8, INO80N, ACTR8, Actin-related protein 8, hArp8, INO80 complex subunit N

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Q9H981

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 99% PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MNHKVMILMKMGFSGIVVHQESVCATYGSGLSSTCIVDVGDQKTSVCCVEDGVSHRNTRIFSWNQDISGLQDHEFQIRHPDSPALLYQFRLGDEKLQAPMALFYPATFGIVGQKMTTLQHRSQGDPEDPHDEHYLLATQSKQEQSAKATADRKSASKPIGFEGDLRGQSSDLPERLHSQEVDLGSAQGDGLMAGNDSEEALTALMSRKTAISLFEGKALGLDKAILHSIDCCSSDDTKKKMYSSILVVGGGLMFHKAQEFLQHRILNKMPPSFRRIIENVDVITRPKDMDPRLIAWKGGAVLACLDTTQELWIYQREWQRFGVRMLRERAAFVW","proteinLength":"Full Length","predictedMolecularWeight":"38.17 kDa","actualMolecularWeight":null,"aminoAcidEnd":329,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H981","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ACTR8 also known as Actin-related protein 8 plays a role in chromatin remodeling. This protein has a molecular mass of approximately 47 kDa. ACTR8 is mostly expressed in cells with high proliferative capacity and functions as a component of the chromatin remodeling SWR1 complex. The SWR1 complex is vital for the exchange of histone variant H2A.Z impacting chromatin structure and gene regulation. ACTR8 has homologous sequences similar to conventional actins but performs distinct functions related to nuclear processes rather than cytoplasmic structure.
Biological function summary

ACTR8 engages in chromatin-related activities to regulate gene expression. It forms part of the SWR1 complex which assembles with other proteins like SWR1 and BAF53A to execute nucleosome shifts. This complex integrates various signals to influence global gene expression patterns necessary for proper cellular function. By modulating access to DNA ACTR8 contributes to pivotal processes like DNA repair and replication highlighting its role beyond mere structural support in chromatin architecture.

Pathways

The actions of ACTR8 influence both the DNA damage response and transcriptional regulation pathways. By incorporating into the SWR1 complex it facilitates the deposition of variant histones essential for directing the DNA damage repair machinery and supporting cell cycle progression. Its activity intersects with pathways involving proteins such as H2A.Z and SWI/SNF making ACTR8 important for maintaining cellular homeostasis and ensuring accurate gene transcriptional dynamics.

ACTR8 impacts cancer progression and developmental disorders. Alterations or misregulation of ACTR8 and its associated SWR1 complex can disrupt normal chromatin dynamics promoting tumorigenesis. Additionally mutations involving ACTR8-linked chromatin remodeling have connections to certain developmental disorders. The interplay between ACTR8 and proteins like H2A.Z highlights its importance in these conditions highlighting potential targets for therapeutic interventions.
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Specifications

Form

Liquid

Additional notes

ab172851 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE. It is supplied as an 0.2 µM filtered solution.

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General info

Function

Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize.. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.

Sequence similarities

Belongs to the actin family. ARP8 subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize.. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.
See full target information ACTR8
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