Recombinant Human ACTR8 protein is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
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| Application | Reactivity | Dilution info | Notes |
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Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Required for the recruitment of INO80 (and probably the INO80 complex) to sites of DNA damage. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.
ARP8, INO80N, ACTR8, Actin-related protein 8, hArp8, INO80 complex subunit N
Recombinant Human ACTR8 protein is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
pH: 7.4
Constituents: 99% PBS, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
ab172851 is greater than 95% pure, as determined by SEC-HPLC and reducing SDS-PAGE. It is supplied as an 0.2 µM filtered solution.
Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize.
Belongs to the actin family. ARP8 subfamily.
ACTR8 also known as Actin-related protein 8 plays a role in chromatin remodeling. This protein has a molecular mass of approximately 47 kDa. ACTR8 is mostly expressed in cells with high proliferative capacity and functions as a component of the chromatin remodeling SWR1 complex. The SWR1 complex is vital for the exchange of histone variant H2A.Z impacting chromatin structure and gene regulation. ACTR8 has homologous sequences similar to conventional actins but performs distinct functions related to nuclear processes rather than cytoplasmic structure.
ACTR8 engages in chromatin-related activities to regulate gene expression. It forms part of the SWR1 complex which assembles with other proteins like SWR1 and BAF53A to execute nucleosome shifts. This complex integrates various signals to influence global gene expression patterns necessary for proper cellular function. By modulating access to DNA ACTR8 contributes to pivotal processes like DNA repair and replication highlighting its role beyond mere structural support in chromatin architecture.
The actions of ACTR8 influence both the DNA damage response and transcriptional regulation pathways. By incorporating into the SWR1 complex it facilitates the deposition of variant histones essential for directing the DNA damage repair machinery and supporting cell cycle progression. Its activity intersects with pathways involving proteins such as H2A.Z and SWI/SNF making ACTR8 important for maintaining cellular homeostasis and ensuring accurate gene transcriptional dynamics.
ACTR8 impacts cancer progression and developmental disorders. Alterations or misregulation of ACTR8 and its associated SWR1 complex can disrupt normal chromatin dynamics promoting tumorigenesis. Additionally mutations involving ACTR8-linked chromatin remodeling have connections to certain developmental disorders. The interplay between ACTR8 and proteins like H2A.Z highlights its importance in these conditions highlighting potential targets for therapeutic interventions.
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