Recombinant Human ACY-1 protein is a Human Full Length protein, in the 1 to 408 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M T S K G P E E E H P S V T L F R Q Y L R I R T V Q P K P D Y G A A V A F F E E T A R Q L G L G C Q K V E V A P G Y V V T V L T W P G T N P T L S S I L L N S H T D V V P V F K E H W S H D P F E A F K D S E G Y I Y A R G A Q D M K C V S I Q Y L E A V R R L K V E G H R F P R T I H M T F V P D E E V G G H Q G M E L F V Q R P E F H A L R A G F A L D E G I A N P T D A F T V F Y S E R S P W W V R V T S T G R P G H A S R F M E D T A A E K L H K V V N S I L A F R E K E W Q R L Q S N P H L K E G S V T S V N L T K L E G G V A Y N V I P A T M S A S F D F R V A P D V D F K A F E E Q L Q S W C Q A A G E G V T L E F A Q K W M H P Q V T P T D D S N P W W A A F S R V C K D M N L T L E P E I M P A A T D N R Y I R A V G V P A L G F S P M N R T P V L L H D H D E R L H E A V F L R G V D I Y T R L L P A L A S V P A L P S D S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.
Alternative names
Aminoacylase-1, ACY-1, N-acyl-L-amino-acid amidohydrolase, ACY1
Recommended products
Recombinant Human ACY-1 protein is a Human Full Length protein, in the 1 to 408 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q03154-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M T S K G P E E E H P S V T L F R Q Y L R I R T V Q P K P D Y G A A V A F F E E T A R Q L G L G C Q K V E V A P G Y V V T V L T W P G T N P T L S S I L L N S H T D V V P V F K E H W S H D P F E A F K D S E G Y I Y A R G A Q D M K C V S I Q Y L E A V R R L K V E G H R F P R T I H M T F V P D E E V G G H Q G M E L F V Q R P E F H A L R A G F A L D E G I A N P T D A F T V F Y S E R S P W W V R V T S T G R P G H A S R F M E D T A A E K L H K V V N S I L A F R E K E W Q R L Q S N P H L K E G S V T S V N L T K L E G G V A Y N V I P A T M S A S F D F R V A P D V D F K A F E E Q L Q S W C Q A A G E G V T L E F A Q K W M H P Q V T P T D D S N P W W A A F S R V C K D M N L T L E P E I M P A A T D N R Y I R A V G V P A L G F S P M N R T P V L L H D H D E R L H E A V F L R G V D I Y T R L L P A L A S V P A L P S D S
- Accession
- Q03154
- Protein length
- Full Length
- Predicted molecular weight
- 48 kDa
- Amino acids
- 1 to 408
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99247 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.
- Sequence similarities
Belongs to the peptidase M20A family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein ACY-1 also known as aminoacylase 1 or ACY plays a significant role in the deacetylation process of N-acyl amino acids. ACY-1 is a zinc-binding enzyme and has a molecular weight of approximately 45 kDa. This enzyme is expressed in various tissues including the liver and kidney. ACY-1 is essential for amino acid metabolism by catalyzing the hydrolysis of N-acyl-L-amino acids into L-amino acids and free fatty acids.
- Biological function summary
The aminoacylase 1 enzyme functions in detoxification and metabolic regulation. It is not typically part of large protein complexes but acts independently to regulate amino acid and acetyl-CoA pathways. The activity facilitates the recycling of acyl groups and amino acids which is important for maintaining cellular homeostasis.
- Pathways
Aminoacylase 1 is involved in amino acid catabolism and lipid metabolism pathways. In these processes ACY-1 interacts with proteins such as acetyl-CoA synthetase in the conversion of excess acyl-CoAs into free fatty acids and CoA preventing the build-up of toxic metabolites. It also has an indirect relationship with the urea cycle by ensuring the availability of free amino acids for further metabolism.
- Associated diseases and disorders
Alterations in ACY-1 function relate to neurological disorders and metabolic syndromes. For instance defects in ACY-1 have been associated with Canavan disease a condition affecting the brain. The protein's activity imbalance might also link to metabolic disorders where its interaction with proteins like aspartoacylase comes into play further affecting the acetylation status and metabolic regulation.
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1 product image
SDS-PAGE - Recombinant Human ACY-1 protein (ab99247)
15% SDS-PAGE using ab99247 (3μg).
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