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AB157892

Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus)

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Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 575 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Acyloxyacyl hydrolase, AOAH

1 Images
SDS-PAGE - Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus) (AB157892)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Acyloxyacyl Hydrolase protein (GST tag N-Terminus) (AB157892)

ab157892 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P28039

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB233405

Anti-Acyloxyacyl Hydrolase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

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  • 0
Primary Antibodies

AB111903

Anti-Adenosyl Homocysteine antibody [EPR4499]

RabMAb|Recombinant

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] (AB111903)

  • 0
  • 0
Primary Antibodies

AB168537

Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free

RabMAb|Recombinant

Competitive ELISA - Anti-Adenosyl Homocysteine antibody [EPR4499] - BSA and Azide free (AB168537)

  • 0
  • 0
Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MQSPWKILTVAPLFLLLSLQSSASPANDDQSRPSLSNGHTCVGCVLVVSVIEQLAQVHNSTVQASMERLCSYLPEKLFLKTTCYLVIDKFGSDIIKLLSADMNADVVCHTLEFCKQNTGQPLCHLYPLPKETWKFTLQKARQIVKKSPILKYSRSGSDICSLPVLAKICQKIKLAMEQSVPFKDVDSDKYSVFPTLRGYHWRGRDCNDSDESVYPGRRPNNWDVHQDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":575,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P28039","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Acyloxyacyl Hydrolase' also known as AOAH is an enzyme that cleaves secondary acyl chains from the lipid A portion of lipopolysaccharides (LPS) on bacterial membranes decreasing their toxicity. This process is known as hydrolasing activity. AOAH is a hydrolase protein with an approximate mass of 54 kDa and is widely expressed in various cells such as macrophages neutrophils and dendritic cells. Its activity is critical in modulating the immune response to LPS by removing these acyl chains which is essential in preventing excessive inflammation.
Biological function summary

Acyloxyacyl Hydrolase plays a significant role in the detoxification of LPS. As part of the host's defense mechanism AOAH works by reducing the endotoxic effects of LPS which are powerful stimulants of the immune system. Although it is not solely part of a larger protein complex its action significantly impacts the innate immune response. This enzyme ensures that the immune system does not overreact to bacterial invasion which could otherwise lead to harmful inflammatory responses.

Pathways

Acyloxyacyl Hydrolase functions within the Toll-like receptor (TLR) signaling pathway which is instrumental in recognizing LPS and initiating an immune response. AOAH modifies LPS diminishing its ability to trigger TLR4 a receptor that responds to the presence of bacterial LPS. By doing so it regulates signaling pathways involved in innate immunity. Additionally it has a relationship with proteins like myeloid differentiation factor 88 (MyD88) an important adaptor protein in the TLR signaling cascade which mediates inflammatory responses.

Acyloxyacyl Hydrolase has connections to sepsis and chronic inflammatory conditions. In sepsis the clearance of LPS by AOAH helps mitigate excessive inflammation reducing the severity of the condition. Hospitals often face challenges in managing sepsis due to overwhelming immune responses where AOAH’s role in detoxifying LPS is critical. Moreover in chronic inflammatory diseases AOAH's activity serves as a checkpoint maintaining inflammation at manageable levels. Its relationship with TLR4 highlights its role in controlling pathways that drive such disorders.
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Specifications

Form

Liquid

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General info

Function

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed : 1883828, PubMed : 29343645, PubMed : 8089145). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).

Post-translational modifications

Cleaved into a large and a small subunit.. The small subunit is N-glycosylated.

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Product protocols

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Target data

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed : 1883828, PubMed : 29343645, PubMed : 8089145). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).
See full target information AOAH
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Product promise

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