Recombinant Human ADAM17 protein is a Human Fragment protein, in the 215 to 671 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Images
Publications
Filter by
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e227342022ADAM10- and γ-secretase-dependent cleavage of the transmembrane protein PTPRT attenuates neurodegeneration in the mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiling Liu,Zhongyu Zhang,Lianwei Li,Li Yao,Zhanshan Ma,Jiali LiPubMed 36583697
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
No
Amino acid sequence
R A D P D P M K N T C K L L V V A D H R F Y R Y M G R G E E S T T T N Y L I E L I D R V D D I Y R N T S W D N A G F K G Y G I Q I E Q I R I L K S P Q E V K P G E K H Y N M A K S Y P N E E K D A W D V K M L L E Q F S F D I A E E A S K V C L A H L F T Y Q D F D M G T L G L A Y V G S P R A N S H G G V C P K A Y Y S P V G K K N I Y L N S G L T S T K N Y G K T I L T K E A D L V T T H E L G H N F G A E H D P D G L A E C A P N E D Q G G K Y V M Y P I A V S G D H E N N K M F S N C S K Q S I Y K T I E S K A Q E C F Q E R S N K V C G N S R V D E G E E C D P G I M Y L N N D T C C N S D C T L K E G V Q C S D R N S P C C K N C Q F E T A Q K K C Q E A I N A T C K G V S Y C T G N S S E C P P P G N A E D D T V C L D L G K C K D G K C I P F C E R E Q Q L E S C A C N E T D N S C K V C C R D L S G R C V P Y V D A E Q K N L F L R K G K P C T V G F C D M N G K C E K R V Q D V I E R F W D F I D Q L S I N T F G K F L A D N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed:26876177, PubMed:28060820).
Alternative names
Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17, TACE, CSVP
Recommended products
Recombinant Human ADAM17 protein is a Human Fragment protein, in the 215 to 671 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
No
- Accession
- P78536-1
- Animal free
Yes
- Carrier free
Yes
- Species
Human
- Reconstitution
Store lyophilized form at room temperature. Reconstitute with PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeat freeze/thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic
Sequence info
Amino acid sequence
R A D P D P M K N T C K L L V V A D H R F Y R Y M G R G E E S T T T N Y L I E L I D R V D D I Y R N T S W D N A G F K G Y G I Q I E Q I R I L K S P Q E V K P G E K H Y N M A K S Y P N E E K D A W D V K M L L E Q F S F D I A E E A S K V C L A H L F T Y Q D F D M G T L G L A Y V G S P R A N S H G G V C P K A Y Y S P V G K K N I Y L N S G L T S T K N Y G K T I L T K E A D L V T T H E L G H N F G A E H D P D G L A E C A P N E D Q G G K Y V M Y P I A V S G D H E N N K M F S N C S K Q S I Y K T I E S K A Q E C F Q E R S N K V C G N S R V D E G E E C D P G I M Y L N N D T C C N S D C T L K E G V Q C S D R N S P C C K N C Q F E T A Q K K C Q E A I N A T C K G V S Y C T G N S S E C P P P G N A E D D T V C L D L G K C K D G K C I P F C E R E Q Q L E S C A C N E T D N S C K V C C R D L S G R C V P Y V D A E Q K N L F L R K G K P C T V G F C D M N G K C E K R V Q D V I E R F W D F I D Q L S I N T F G K F L A D N
- Accession
- Protein length
Fragment
- Predicted molecular weight
51.22 kDa
- Actual molecular weight
51.23 kDa
- Amino acids
215 to 671
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
>95% purity determined by SDS-PAGE and HPLC.
General info
- Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed:26876177, PubMed:28060820).
- Post-translational modifications
The precursor is cleaved by a furin endopeptidase.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
Notes
For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
- Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
- Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
- Associated diseases and disorders
ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human ADAM17 protein (ab282373)
SDS-PAGE analysis of ab282373
Mass Spectrometry - Recombinant Human ADAM17 protein (ab282373)
Mass determination by ESI-TOF.
Predicted MW 51227.47 is Da (+/- 10 Da by ESI-TOF). Observed MW is 51234.62 Da.
HPLC - Recombinant Human ADAM17 protein (ab282373)
HPLC analysis of ab282373.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com