Recombinant Human ADAM23 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
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Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
sELISA, SDS-PAGE
- Biologically active
No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.
Alternative names
Disintegrin and metalloproteinase domain-containing protein 23, ADAM 23, MDC-3, ADAM23, MDC3
Recommended products
Recombinant Human ADAM23 Protein Standard (His tag) is a Human Fragment protein, expressed in HEK 293, with >=80% purity and suitable for sELISA, SDS-PAGE.
Alternative names
Disintegrin and metalloproteinase domain-containing protein 23, ADAM 23, MDC-3, ADAM23, MDC3
Key facts
- Purity
>=80% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
sELISA, SDS-PAGE
- Biologically active
No
- Accession
- O75077-1
- Animal free
Yes
- Species
Human
- Concentration
- Storage buffer
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- Accession
- Protein length
Fragment
- Predicted molecular weight
61 kDa
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Liquid
General info
- Function
May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM23 also known as A Disintegrin And Metalloprotease 23 is a member of the ADAM family of proteases. This protein has a mass of approximately 95 kDa. ADAM23 is expressed in several tissues but it has a significant presence in the brain. The protein mechanically functions by engaging in protease activities though it is uniquely categorized due to its role beyond traditional proteolytic functions. It interacts with surrounding cell-matrix elements through disintegrin domains.
- Biological function summary
ADAM23 plays a role in processes such as cell-cell adhesion and signal transduction. It aids in the regulation of synapse formation and maintenance in neuronal cells which is important for proper neural development and function. ADAM23 is not generally considered a part of large protein complexes but collaborates in the cellular environment impacting spatial cellular frameworks and growth signaling cascades.
- Pathways
Multiple mechanisms rely on ADAM23 especially those involved in cell signaling. The protein interacts with integrin pathways influencing cellular adhesion and migration dynamics. Furthermore it works closely with other members of the ADAM family such as ADAM22 in modulating signaling pathways critical for neuronal function and transmission.
- Associated diseases and disorders
ADAM23 has shown connections to neurological conditions notably epilepsy and some types of cancer. Disruptions or mutations in the ADAM23 gene have associated alterations in nervous system activity contributing to epilepsy pathogenesis. During cancer progression ADAM23 may interact with tumor-suppressing pathways in which its family member ADAM22 also participates. Understanding ADAM23's role in these diseases can lead to new insights into therapeutic targets.
Product promise
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2 product images
Sandwich ELISA - Recombinant Human ADAM23 Protein Standard (His tag) (ab319026)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
SDS-PAGE - Recombinant Human ADAM23 Protein Standard (His tag) (ab319026)
SDS-PAGE analysis of ab319026 under reducing conditions for 2ug protein.
Downloads
Product protocols
- Visit the general protocols
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