Recombinant human ADAMTS1 protein is a Human Fragment protein, in the 253 to 616 aa range, expressed in Baculovirus infected insect, with >90% purity and suitable for SDS-PAGE, Inhib, FuncS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, Inhib, FuncS
- Biologically active
- Yes
Amino acid sequence
F V S S H R Y V E T M L V A D Q S M A E F H G S G L K H Y L L T L F S V A A R L Y K H P S I R N S V S L V V V K I L V I H D E Q K G P E V T S N A A L T L R N F C N W Q K Q H N P P S D R D A E H Y D T A I L F T R Q D L C G S Q T C D T L G M A D V G T V C D P S R S C S V I E D D G L Q A A F T T A H E L G H V F N M P H D D A K Q C A S L N G V N Q D S H M M A S M L S N L D H S Q P W S P C S A Y M I T S F L D N G H G E C L M D K P H N P I Q L P G D L P G T S Y D A N R Q C Q F T F G E D S K H C P D A A S T C S T L W C T G T S G G V L V C Q T K H F P W A D G T S C G E G K W C I N G K C V N K T D R K H F D T P F H G N W G M W G P W G D C S R T C G G G V Q Y T M R E C D N P V P K N G G K Y C E G K R V R Y R S C N L E D C P D N ( H ) < s u b > 6 < / s u b >
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application Inhib | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
Alternative names
KIAA1346, METH1, ADAMTS1, A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1
Recommended products
Recombinant human ADAMTS1 protein is a Human Fragment protein, in the 253 to 616 aa range, expressed in Baculovirus infected insect, with >90% purity and suitable for SDS-PAGE, Inhib, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, Inhib, FuncS
- Biologically active
- Yes
- Biological activity
- Activity of ab134430 is determined with recombinant aggrecan interglobular domain. ab134430 hydrolyzes the aggrecanase site within this domain (peptide bond E373 - A374 in Human aggrecan). The recombinant substrate is incubated at a concentration of 0.1 μM with 0.2-3.0 nM ADAMTS1 in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2, 1 μM leupeptin, 1 μM pepstatin, 1 mM Pefabloc, 0.05 % Brij 35 for 15 min at 37°C. Cleavage at the aggrecanase-site is estimated from the appearance of the hydrolysis fragment with the novel N-terminus ARGSVIL. The fragment is quantified with two monoclonal antibodies, one directed against the neoepitope ARGSVIL, the other against the sequence C-terminal to the neoepitope. Under the specified conditions the hydrolysis rate is > 0.06 nM hydrolysed substrate/min x nM truncated ADAMTS1. When related to mg enzyme, the value is > 1.4 nmoles hydrolyzed substrate/min x mg ADAMTS1.
- Accession
- Q9UHI8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 0.88% Sodium chloride, 0.79% Tris HCl, 0.05% Calcium chloride, 0.05% Polyoxyethylene lauryl ether
Sequence info
Amino acid sequence
- F V S S H R Y V E T M L V A D Q S M A E F H G S G L K H Y L L T L F S V A A R L Y K H P S I R N S V S L V V V K I L V I H D E Q K G P E V T S N A A L T L R N F C N W Q K Q H N P P S D R D A E H Y D T A I L F T R Q D L C G S Q T C D T L G M A D V G T V C D P S R S C S V I E D D G L Q A A F T T A H E L G H V F N M P H D D A K Q C A S L N G V N Q D S H M M A S M L S N L D H S Q P W S P C S A Y M I T S F L D N G H G E C L M D K P H N P I Q L P G D L P G T S Y D A N R Q C Q F T F G E D S K H C P D A A S T C S T L W C T G T S G G V L V C Q T K H F P W A D G T S C G E G K W C I N G K C V N K T D R K H F D T P F H G N W G M W G P W G D C S R T C G G G V Q Y T M R E C D N P V P K N G G K Y C E G K R V R Y R S C N L E D C P D N ( H ) < s u b > 6 < / s u b >
- Accession
- Q9UHI8
- Protein length
- Fragment
- Predicted molecular weight
- 41 kDa
- Amino acids
- 253 to 616
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab134430 was purified from insect cell culture supernatants by affinity purification.
General info
- Function
Cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
- Post-translational modifications
The precursor is cleaved by a furin endopeptidase.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAMTS1 short for a disintegrin and metalloproteinase with thrombospondin motifs 1 is an enzyme with a mass of around 110 kDa. Researchers often study this enzyme for its role in protein degradation and modification. ADAMTS1 is found in various tissues including the heart lungs and kidneys. This protein has several aliases sometimes referred to as ADAMTS-1 or ADAM-TS1. Its primary function involves processing extracellular matrix components such as aggrecan and versican which contribute to tissue remodeling.
- Biological function summary
The influence of ADAMTS1 extends to various physiological and developmental processes. This enzyme participates in the modulation of angiogenesis inflammation and reproductive functions. It operates within a complex of metalloproteinases coordinating the breakdown of matrix proteins. Through these activities ADAMTS1 maintains tissue integrity and homeostasis. It particularly plays an essential role in the regulation of proteoglycans which are critical in cellular communication and structure.
- Pathways
The actions of ADAMTS1 intersect within the extracellular matrix organization and integrin signaling pathways. These pathways are important for cell adhesion and migration contributing to tissue morphogenesis and repair. ADAMTS1 shows interaction with proteins like integrins and other matrix metalloproteinases establishing a network critical for cellular structural changes and signal transduction. These interactions ensure balanced tissue development and response to environmental changes.
- Associated diseases and disorders
The dysfunction of ADAMTS1 has links to conditions like osteoarthritis and cancer. In osteoarthritis inadequate regulation of matrix components by ADAMTS1 can lead to joint degradation. In cancer its dysregulation may influence tumor growth and metastasis. ADAMTS1 interacts with other proteins implicated in these diseases such as aggrecanases in osteoarthritis and integrins in cancer. These connections highlight the potential role of ADAMTS1 as a therapeutic target in managing these complex diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human ADAMTS1 protein (ab134430)
SDS-PAGE analysis of ab134430 (2 μg)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com