Recombinant Human ADAT2 protein is a Human Full Length protein, in the 1 to 191 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E A K A A P K P A A S G A C S V S A E E T E K W M E E A M H M A K E A L E N T E V P V G C L M V Y N N E V V G K G R N E V N Q T K N A T R H A E M V A I D Q V L D W C R Q S G K S P S E V F E H T V L Y V T V E P C I M C A A A L R L M K I P L V V Y G C Q N E R F G G C G S V L N I A S A D L P N T G R P F Q C I P G Y R A E E A V E M L K T F Y K Q E N P N A P K S K V R K K E C Q K S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probably participates in deamination of adenosine-34 to inosine in many tRNAs.
Alternative names
DEADC1, ADAT2, tRNA-specific adenosine deaminase 2, Deaminase domain-containing protein 1, tRNA-specific adenosine-34 deaminase subunit ADAT2
Recommended products
Recombinant Human ADAT2 protein is a Human Full Length protein, in the 1 to 191 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q7Z6V5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E A K A A P K P A A S G A C S V S A E E T E K W M E E A M H M A K E A L E N T E V P V G C L M V Y N N E V V G K G R N E V N Q T K N A T R H A E M V A I D Q V L D W C R Q S G K S P S E V F E H T V L Y V T V E P C I M C A A A L R L M K I P L V V Y G C Q N E R F G G C G S V L N I A S A D L P N T G R P F Q C I P G Y R A E E A V E M L K T F Y K Q E N P N A P K S K V R K K E C Q K S
- Accession
- Q7Z6V5
- Protein length
- Full Length
- Predicted molecular weight
- 23.2 kDa
- Amino acids
- 1 to 191
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Probably participates in deamination of adenosine-34 to inosine in many tRNAs.
- Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAT2 also known as ADAT2 deaminase plays an important role in RNA editing by converting adenosines to inosines in tRNA molecules. This deaminase has a mass of roughly 50 kDa and is widely expressed in various tissues. Its presence is important for maintaining RNA stability and accurate coding which together plays pivotal part in protein synthesis.
- Biological function summary
ADAT2 works as part of the ADAT complex that includes ADAT3. This complex specifically targets anticodon regions of tRNAs. The conversion of adenosine to inosine by ADAT2 changes the reading of certain codons allowing for proper interpretation of genetic code elements important for cell function and viability. ADAT2’s activity impacts the modification process essential for accurate protein translation.
- Pathways
Four important mechanisms involve ADAT2 in RNA metabolic processes. It plays an integral role in the post-transcriptional modification of RNA particularly within the tRNA maturation pathway. This pathway collaborates with proteins such as ADAT3 and their interaction governs fidelity in transcribing genetic information into proteins. The editing by ADAT2 ensures that tRNA molecules can correctly interpret codons during translation.
- Associated diseases and disorders
Four recognized conditions involve faulty ADAT2 activity: one is neurodevelopmental disorders where genetic mutations affecting its function impact brain development. Another is some types of cancer where RNA editing disruptions play part in tumorigenic processes. These diseases can involve interactions with other proteins like ADAT3 that need for proper RNA editing balance. Understanding these interactions may help in developing therapeutic strategies targeting RNA editing mechanisms.
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1 product image
SDS-PAGE - Recombinant Human ADAT2 protein (ab101153)
ab101153 used in 15% SDS-PAGE (3 μg)
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