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AB191461

Recombinant Human ADCK3 protein

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Recombinant Human ADCK3 protein is a Human Fragment protein, in the 156 to 647 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE.

View Alternative Names

ADCK3, CABC1, PP265, COQ8A, Chaperone activity of bc1 complex-like, Coenzyme Q protein 8A, aarF domain-containing protein kinase 3, Chaperone-ABC1-like

1 Images
SDS-PAGE - Recombinant Human ADCK3 protein (AB191461)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ADCK3 protein (AB191461)

The purity of ab191461 was determined to be >75% by densitometry. Approximate MW is ~83 kDa.

Key facts

Purity

>75% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8NI60

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Primary Antibodies

AB230897

Anti-ADCK3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADCK3 antibody (AB230897)

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Proteins & Peptides

AB89485

Recombinant Human Estrogen Inducible Protein pS2

SDS-PAGE - Recombinant Human Estrogen Inducible Protein pS2 (AB89485)

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Proteins & Peptides

AB191463

Recombinant Human ADCK5 protein

SDS-PAGE - Recombinant Human ADCK5 protein (AB191463)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MGFQRRFFHQDQSPVGGLTAEDIEKARQAKARPENKQHKQTLSEHARERKVPVTRIGRLANFGGLAVGLGFGALAEVAKKSLRSEDPSGKKAVLGSSPFLSEANAERIVRTLCKVRGAALKLGQMLSIQDDAFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNNLMAVLNMSNMLPEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGYEVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLVPPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEEAYSNYCKRQAQQ","proteinLength":"Fragment","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":647,"aminoAcidStart":156,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q8NI60","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADCK3 also known as AarF domain-containing kinase 3 or COQ8A is a protein kinase with an approximate mass of 68 kDa. It is largely expressed in the mitochondria which indicates its role in cellular energy processes. The human ADCK3 gene encodes this protein and it serves a critical function in the biosynthesis of coenzyme Q10 an essential component of the mitochondrial electron transport chain. ADCK3's structure includes a kinase domain hinting at its enzymatic activity in phosphorylation processes.
Biological function summary

ADCK3 participates in the regulation of coenzyme Q10 levels an essential lipid-soluble antioxidant. The protein is a part of the mitochondrial electron transport chain complex contributing to the production of ATP. As a kinase ADCK3 influences the modulation of the complex's components through phosphorylation stabilizing the function and efficiency of energy conversion processes within mitochondria. Its role is critical for maintaining cellular energy balance and reducing oxidative stress.

Pathways

ADCK3 engages in the coenzyme Q10 biosynthesis pathway. It closely interacts with COQ proteins like COQ9 orchestrating the synthesis and function of the electron transport chain. Additionally it takes part in mitochondrial kinase pathways that ensure proper mitochondrial function. The cooperation between ADCK3 and other proteins such as COQ6 emphasizes its importance in maintaining mitochondrial integrity and energy metabolism.

Mutations in the ADCK3 gene have been linked to Coenzyme Q10 deficiency and spinocerebellar ataxia. Individuals with mutations often face progressive cerebellar atrophy a condition that affects motor skills and movement coordination. The association of ADCK3 with COQ6 in these disorders highlights its influence in the pathological development and underlines the potential therapeutic interest in modulating its activity.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PubMed : 21296186, PubMed : 25498144, PubMed : 25540914, PubMed : 27499294, PubMed : 36302899, PubMed : 38425362). Its substrate specificity is still unclear : may act as a protein kinase that mediates phosphorylation of COQ3 (By similarity). According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates (PubMed : 25498144, PubMed : 27499294). However, the small molecule kinase activity was not confirmed by another publication (By similarity). Shows an unusual selectivity for binding ADP over ATP (PubMed : 25498144).

Sequence similarities

Belongs to the protein kinase superfamily. ADCK protein kinase family.

Subcellular localisation

Mitochondrion membrane

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Product protocols

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Target data

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PubMed : 21296186, PubMed : 25498144, PubMed : 25540914, PubMed : 27499294, PubMed : 36302899, PubMed : 38425362). Its substrate specificity is still unclear : may act as a protein kinase that mediates phosphorylation of COQ3 (By similarity). According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates (PubMed : 25498144, PubMed : 27499294). However, the small molecule kinase activity was not confirmed by another publication (By similarity). Shows an unusual selectivity for binding ADP over ATP (PubMed : 25498144).
See full target information COQ8A
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