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AB132569

Recombinant Human ADH4 protein

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Recombinant Human ADH4 protein is a Human Full Length protein, in the 1 to 380 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

ADH2, ADH4, All-trans-retinol dehydrogenase [NAD(+)] ADH4, Alcohol dehydrogenase 2, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain

1 Images
SDS-PAGE - Recombinant Human ADH4 protein (AB132569)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADH4 protein (AB132569)

12.5% SDS-PAGE analysis of ab132569 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P08319

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGTKGKVIKCKAAIAWEAGKPLCIEEVEVAPPKAHEVRIQIIATSLCHTDATVIDSKFEGLAFPVIVGHEAAGIVESIGPGVTNVKPGDKVIPLYAPLCRKCKFCLSPLTNLCGKISNLKSPASDQQLMEDKTSRFTCKGKPVYHFFGTSTFSQYTVVSDINLAKIDDDANLERVCLLGCGFSTGYGAAINNAKVTPGSTCAVFGLGGVGLSAVMGCKAAGASRIIGIDINSEKFVKAKALGATDCLNPRDLHKPIQEVIIELTKGGVDFALDCAGGSETMKAALDCTTAGWGSCTFIGVAAGSKGLTVFPEELIIGRTINGTFFGGWKSVDSIPKLVTDYKNKKFNLDALVTHTLPFDKISEAFDLMNQGKSIRTILIF","proteinLength":"Full Length","predictedMolecularWeight":"66.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P08319","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADH4 also known as alcohol dehydrogenase 4 is an enzyme with a mass of approximately 40 kDa. This enzyme plays a role in the oxidation of alcohols within the digestive system. Not just limited to the digestive system ADH4 also shows expression in the liver and kidneys where it contributes to the metabolic processes. Its action involves converting ethanol and other alcohols to their corresponding aldehydes which are then further metabolized.
Biological function summary

The enzyme participates in the oxidation of retinol to retinoic acid a process essential for diverse biological functions. While ADH4 operates primarily as a standalone enzyme it still interacts with other members of the alcohol dehydrogenase family within the liver. These interactions facilitate intermediary metabolism and help maintain homeostasis. The enzyme's role extends beyond simple alcohol metabolism implicating it in broader metabolic contexts.

Pathways

ADH4 occupies a definite position in the ethanol metabolism pathway. It cooperates with enzymes like aldehyde dehydrogenase to process ethanol into acetic acid a step important for energy production and detoxification. Additionally its activity relates to the retinoic acid signaling pathway. Through this pathway it interacts indirectly with enzymes that process retinoids connecting it to cellular differentiation and vision.

ADH4 has associations with alcohol-related liver disease and fetal alcohol syndrome. In liver disease ADH4's excessive ethanol oxidation can lead to toxic aldehyde accumulation contributing to tissue damage. Its interaction with aldehyde dehydrogenase becomes significant as any dysfunction in this relation might exacerbate disease severity. Moreover disruptions in retinoic acid metabolism where ADH4 plays a part can link to disorders affecting embryonic development highlighting its role in conditions like fetal alcohol syndrome.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol (PubMed : 17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed : 16081420). Also catalyzes the reduction of benzoquinones (PubMed : 10514444).

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Class-II subfamily.

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Product protocols

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Target data

Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol (PubMed : 17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed : 16081420). Also catalyzes the reduction of benzoquinones (PubMed : 10514444).
See full target information ADH4
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