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AB140543

Recombinant Human ADI1 protein

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Recombinant Human ADI1 protein is a Human Full Length protein, in the 1 to 179 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

MTCBP1, HMFT1638, ADI1, Acireductone dioxygenase, Acireductone dioxygenase (Fe(2+)-requiring), Acireductone dioxygenase (Ni(2+)-requiring), Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor, ARD', Fe-ARD, ARD, Ni-ARD, MTCBP-1, Sip-L

1 Images
SDS-PAGE - Recombinant Human ADI1 protein (AB140543)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADI1 protein (AB140543)

15% SDS-PAGE analysis of ab140543 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9BV57

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSSMVLAWYMDDAPGDPRQPHRPDPGRPVGLEQLRRLGVLYWKLDADKYENDPELEKIRRERNYSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFMEKGDMVTLPAGIYHRFTVDEKNYTKAMRLFVGEPVWTAYNRPADHFEARGQYVKFLAQTA","proteinLength":"Full Length","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":179,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BV57","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADI1 also known as aci-reductone dioxygenase 1 is an enzyme involved in the methionine salvage pathway. Its molecular mass is approximately 26 kDa and it is mainly expressed in the liver and kidney tissues. Mechanically ADI1 functions as a metal-dependent dioxygenase that catalyzes the oxidation of the aci-reductone substrate to produce formate and carbon monoxide. This step is essential in recycling methionine from methylthioadenosine which is a significant component of the metabolic process.
Biological function summary

ADI1 plays an important role in cellular metabolism especially in the regulation of methionine levels. By participating in the methionine salvage pathway it supports the maintenance of cellular methylation potential and antioxidant defense. As a stand-alone enzyme ADI1 does not form part of a larger complex but interacts with other enzymes in the salvage pathway system to ensure efficient regeneration of methionine.

Pathways

ADI1 takes part in the methionine salvage pathway and indirectly influences the broader methionine cycle. This pathway interconnects with important cellular processes such as transmethylation where SAM (S-adenosylmethionine)-dependent methylation reactions occur. Through its role in methionine salvage ADI1 is associated with proteins like MAT (methionine adenosyltransferase) that are important for the biosynthesis of SAM. These interactions integrate ADI1 within the cellular economy of sulfur-containing amino acids and related metabolic activities.

ADI1 relates to several conditions where methionine metabolism is disrupted such as liver diseases and certain types of cancer. Its role in sustaining methionine levels makes it significant in contexts where amino acid balance is critical. For example with liver disease perturbation in methionine recycling can lead to metabolic imbalances. Furthermore ADI1’s activity connects to the MAT enzyme implicated in the regulation of SAM levels an important factor in both the promotion and inhibition of tumor development. ADI1 through the maintenance of methionine homeostasis may play a part in disease pathways involving oxidative stress and methylation dysregulation.
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Specifications

Form

Liquid

Additional notes

ab140543 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site (By similarity). Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway (PubMed : 15938715). Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity). Also down-regulates cell migration mediated by MMP14 (PubMed : 14718544). Necessary for hepatitis C virus replication in an otherwise non-permissive cell line (PubMed : 11602742).

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site (By similarity). Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway (PubMed : 15938715). Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity). Also down-regulates cell migration mediated by MMP14 (PubMed : 14718544). Necessary for hepatitis C virus replication in an otherwise non-permissive cell line (PubMed : 11602742).
See full target information ADI1
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