Recombinant human Adiponectin protein is a Human Full Length protein, in the 16 to 244 aa range, expressed in HEK 293, with >=90% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=90% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
E T T T Q G P G V L L P L P K G A C T G W M A G I P G H P G H N G A P G R D G R D G T P G E K G E K G D P G L I G P K G D I G E T G V P G A E G P R G F P G I Q G R K G E P G E G A Y V Y R S A F S V G L E T Y V T I P N M P I R F T K I F Y N Q Q N H Y D G S T G K F H C N I P G L Y Y F A Y H I T V Y M K D V K V S L F K K D K A M L F T Y D Q Y Q E N N V D Q A S G S V L L H L E V G D Q V W L Q V Y G E G E R N G L Y A D N D N D S T F T G F L L Y H D T N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Alternative names
ACDC, ACRP30, APM1, GBP28, ADIPOQ, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, apM-1
Recommended products
Recombinant human Adiponectin protein is a Human Full Length protein, in the 16 to 244 aa range, expressed in HEK 293, with >=90% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Activates AMPK.
- Accession
- Q15848-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in 50 µL of water
- Concentration
- Storage buffer
Constituents: 99% PBS
Sequence info
Amino acid sequence
- E T T T Q G P G V L L P L P K G A C T G W M A G I P G H P G H N G A P G R D G R D G T P G E K G E K G D P G L I G P K G D I G E T G V P G A E G P R G F P G I Q G R K G E P G E G A Y V Y R S A F S V G L E T Y V T I P N M P I R F T K I F Y N Q Q N H Y D G S T G K F H C N I P G L Y Y F A Y H I T V Y M K D V K V S L F K K D K A M L F T Y D Q Y Q E N N V D Q A S G S V L L H L E V G D Q V W L Q V Y G E G E R N G L Y A D N D N D S T F T G F L L Y H D T N
- Accession
- Q15848
- Protein length
- Full Length
- Predicted molecular weight
- 33 kDa
- Amino acids
- 16 to 244
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
- Post-translational modifications
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.
- Biological function summary
Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.
- Pathways
Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.
- Associated diseases and disorders
Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.
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1 product image
Functional Studies - Recombinant human Adiponectin protein (ab157078)
Size exclusion chromatographyof ab157078. The different forms of adiponectin are indicated by arrows: High molecular weight (HMW; representing 12-18-mer species) and hexamer. The red arrow indicates were one should elute the trimeric state of adiponectin.
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