Recombinant Human Adipose Triglyceride Lipase protein (His tag) is a Human Full Length protein, in the 1 to 504 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M F P R E K T W N I S F A G C G F L G V Y Y V G V A S C L R E H A P F L V A N A T H I Y G A S A G A L T A T A L V T G V C L G E A G A K F I E V S K E A R K R F L G P L H P S F N L V K I I R S F L L K V L P A D S H E H A S G R L G I S L T R V S D G E N V I I S H F N S K D E L I Q A N V C S G F I P V Y C G L I P P S L Q G V R Y V D G G I S D N L P L Y E L K N T I T V S P F S G E S D I C P Q D S S T N I H E L R V T N T S I Q F N L R N L Y R L S K A L F P P E P L V L R E M C K Q G Y R D G L R F L Q R N G L L N R P N P L L A L P P A R P H G P E D K D Q A V E S A Q A E D Y S Q L P G E D H I L E H L P A R L N E A L L E A C V E P T D L L T T L S N M L P V R L A T A M M V P Y T L P L E S A L S F T I R L L E W L P D V P E D I R W M K E Q T G S I C Q Y L V M R A K R K L G R H L P S R L P E Q V E L R R V Q S L P S V P L S C A A Y R E A L P G W M R N N L S L G D A L A K W E E C Q R Q L L L G L F C T N V A F P P E A L R M R A P A D P A P A P A D P A S P Q H Q L A G P A P L L S T P A P E A R P V I G A L G L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674, PubMed:15364929, PubMed:16150821). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone (By similarity). Also has acylglycerol transacylase activity. Acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed:17603008). Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (By similarity).
Alternative names
Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Calcium-independent phospholipase A2, Desnutrin, IPLA2-zeta, Pigment epithelium-derived factor, TTS2.2, Transport-secretion protein 2, TTS2, FP17548, ATGL, PNPLA2
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Recombinant Human Adipose Triglyceride Lipase protein (His tag) is a Human Full Length protein, in the 1 to 504 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q96AD5-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M F P R E K T W N I S F A G C G F L G V Y Y V G V A S C L R E H A P F L V A N A T H I Y G A S A G A L T A T A L V T G V C L G E A G A K F I E V S K E A R K R F L G P L H P S F N L V K I I R S F L L K V L P A D S H E H A S G R L G I S L T R V S D G E N V I I S H F N S K D E L I Q A N V C S G F I P V Y C G L I P P S L Q G V R Y V D G G I S D N L P L Y E L K N T I T V S P F S G E S D I C P Q D S S T N I H E L R V T N T S I Q F N L R N L Y R L S K A L F P P E P L V L R E M C K Q G Y R D G L R F L Q R N G L L N R P N P L L A L P P A R P H G P E D K D Q A V E S A Q A E D Y S Q L P G E D H I L E H L P A R L N E A L L E A C V E P T D L L T T L S N M L P V R L A T A M M V P Y T L P L E S A L S F T I R L L E W L P D V P E D I R W M K E Q T G S I C Q Y L V M R A K R K L G R H L P S R L P E Q V E L R R V Q S L P S V P L S C A A Y R E A L P G W M R N N L S L G D A L A K W E E C Q R Q L L L G L F C T N V A F P P E A L R M R A P A D P A P A P A D P A S P Q H Q L A G P A P L L S T P A P E A R P V I G A L G L
- Accession
- Protein length
Full Length
- Predicted molecular weight
71.3 kDa
- Amino acids
1 to 504
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674, PubMed:15364929, PubMed:16150821). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone (By similarity). Also has acylglycerol transacylase activity. Acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (By similarity). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed:17603008). Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (By similarity).
- Post-translational modifications
Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.
- Biological function summary
ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.
- Pathways
ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.
- Associated diseases and disorders
ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.
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1 product image
SDS-PAGE - Recombinant Human Adipose Triglyceride Lipase protein (His tag) (ab226233)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226233 with 5% enrichment gel and 15% separation gel.
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