JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB95495

Recombinant Human ADK protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ADK protein is a Human Fragment protein, in the 22 to 362 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK

1 Images
SDS-PAGE - Recombinant Human ADK protein (AB95495)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ADK protein (AB95495)

SDS-PAGE analysis of ab95495 (3µg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P55263

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMRENILFGMGNPLLDISAVVDKDFLDKYSLKPNDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKRKAAEAHVDAHYYEQNEQPTGTCAACITGDNRSLIANLAAANCYKKEKHLDLEKNWMLVEKARVCYIAGFFLTVSPESVLKVAHHASENNRIFTLNLSAPFISQFYKESLMKVMPYVDILFGNETEAATFAREQGFETKDIKEIAKKTQALPKMNSKRQRIVIFTQGRDDTIMATESEVTAFAVLDQDQKEIIDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASIIIRRTGCTFPEKPDFH","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":362,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P55263","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine kinase (ADK) also known by its alternative name ATP:adenosine 5'-phosphotransferase is an enzyme with a molecular mass of approximately 40 kDa. This enzyme catalyzes the transfer of a phosphate group from ATP to adenosine forming AMP. ADK is expressed in several tissues with significant presence in the liver brain and lungs. This distribution suggests its versatile role in organ-specific metabolism and regulatory processes.
Biological function summary

The enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.

Pathways

The activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.

Irregularities in ADK function have implications in neurological disorders and liver diseases. Abnormal ADK activity links to epilepsy due to its role in controlling adenosine concentrations affecting neurotransmitter release. Liver diseases such as hepatic steatosis have shown associations with altered ADK function impacting energy balance. The interplay with proteins such as AMP-activated protein kinase indicates potential therapeutic targets in disorders where metabolic regulation is compromised.
style
left-column

Specifications

Form

Liquid

Additional notes

ab95495 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
See full target information ADK
style
left-column
style
left-column
style
right-column

Alternative Products

Proteins & Peptides

AB227392

Recombinant human ADK protein (Active)

SDS-PAGE - Recombinant human ADK protein (Active) (AB227392)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB243636

Anti-ADK antibody [EPR23166-143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] (AB243636)

  • 0
  • 0
Proteins & Peptides

AB280330

Recombinant human Apolipoprotein E3

Mass Spectrometry - Recombinant human Apolipoprotein E3 (AB280330)

  • 0
  • 0
Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

  • 5
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com