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AB130031

Recombinant Human ADPRH protein (His tag N-Terminus)

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Recombinant Human ADPRH protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 357 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ARH1, ADPRH, ADP-ribosylhydrolase ARH1, ADP-ribose-L-arginine cleaving enzyme, [Protein ADP-ribosylarginine] hydrolase, ADP-ribosylarginine hydrolase, hARH1

1 Images
SDS-PAGE - Recombinant Human ADPRH protein (His tag N-Terminus) (AB130031)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADPRH protein (His tag N-Terminus) (AB130031)

15% SDS-PAGE analysis of 3 μg ab130031.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P54922

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMEKYVAAMVLSAAGDALGYYNGKWEFLQDGEKIHRQLAQLGGLDALDVGRWRVSDDTVMHLATAEALVEAGKAPKLTQLYYLLAKHYQDCMEDMDGRAPGGASVHNAMQLKPGKPNGWRIPFNSHEGGCGAAMRAMCIGLRFPHHSQLDTLIQVSIESGRMTHHHPTGYLGALASALFTAYAVNSRPPLQWGKGLMELLPEAKKYIVQSGYFVEENLQHWSYFQTKWENYLKLRGILDGESAPTFPESFGVKERDQFYTSLSYSGWGGSSGHDAPMIAYDAVLAAGDSWKELAHRAFFHGGDSDSTAAIAGCWWGVMYGFKGVSPSNYEKLEYRNRLEETARALYSLGSKEDTVISL","proteinLength":"Full Length","predictedMolecularWeight":"42.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":357,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P54922","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ADP-ribosylarginine hydrolase (ADPRH) is an enzyme that plays a significant role in the reversible modification of target proteins. This enzyme specifically catalyzes the removal of ADP-ribose moieties from arginine residues in proteins a process essential for maintaining protein function. ADPRH is also known by the alternative name ARH1 and has a reported molecular mass of approximately 39 kDa. This enzyme is expressed in various tissues with notable expression in the liver and kidney.
Biological function summary

ADP-ribosylarginine hydrolase participates in cellular regulation processes by modulating protein function through ADP-ribosylation. This modification acts as a switch for protein activity affecting functions like DNA repair and signal transduction. ADPRH does not work in isolation; it forms part of a multi-protein complex that regulates ADP-ribosylation cycles ensuring proper cellular responses to internal and external signals.

Pathways

ADP-ribosylarginine hydrolase is involved in the poly (ADP-ribose) polymerase (PARP) pathway and plays an important role in DNA damage response. The PARP pathway is essential for efficient DNA repair mechanisms and ADPRH helps in the balance of this process alongside PARP1 a well-known related enzyme in the pathway. Also ADPRH's role in pathway dynamics includes its interaction with proteins like PARG which further modulates the ADP-ribosylation process.

ADP-ribosylarginine hydrolase influences conditions associated with impaired DNA repair mechanisms such as cancer and neurodegeneration disorders. Mutations or dysregulation of ADPRH can lead to improper protein function contributing to disease development. ADPRH interacts with PARP1 in cancer where alterations in ADP-ribosylation affect tumor progression. Additionally in neurodegenerative diseases such disruption can impact neuronal survival and function implicating ADPRH in conditions like Parkinson's disease.
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Specifications

Form

Liquid

Additional notes

ab130031 was purified using conventional chromatography techniques.

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General info

Function

Specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.

Sequence similarities

Belongs to the ADP-ribosylglycohydrolase family.

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Product protocols

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Target data

Specifically acts as an arginine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to arginine residues on proteins.
See full target information ADPRH
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