Recombinant Human Adrenomedullin/ADM protein (Fc Chimera) is a Human Fragment protein, in the 95 to 146 aa range, expressed in HEK 293, with >93% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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- Acta biomaterialia 145:146-1582022Nanoparticles with dense poly(ethylene glycol) coatings with near neutral charge are maximally transported across lymphatics and to the lymph nodes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacob McCright,Colin Skeen,Jenny Yarmovsky,Katharina MaiselPubMed 35381399
Key facts
- Purity
- >93% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
Y R Q S M N N F Q G L R S F G C R F G T C T V Q K L A H Q I Y Q F T D K D K D N V A P R S K I S P Q G Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adrenomedullin/ADM and proadrenomedullin N-20 terminal peptide/PAMP are peptide hormones that act as potent hypotensive and vasodilatator agents (PubMed:8387282, PubMed:9620797). Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, ADM is diuretic and natriuretic, and both ADM and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Adrenomedullin. ADM function is mediated by the CALCRL-RAMP2 and CALCRL-RAMP3 receptor complexes with ADM showing the highest potency for the CALCRL-RAMP2 complex.
Alternative names
AM, ADM, Pro-adrenomedullin
Recommended products
Recombinant Human Adrenomedullin/ADM protein (Fc Chimera) is a Human Fragment protein, in the 95 to 146 aa range, expressed in HEK 293, with >93% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >93% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P35318-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- Y R Q S M N N F Q G L R S F G C R F G T C T V Q K L A H Q I Y Q F T D K D K D N V A P R S K I S P Q G Y
- Accession
- P35318
- Protein length
- Fragment
- Predicted molecular weight
- 38 kDa
- Amino acids
- 95 to 146
- Nature
- Recombinant
- Tags
- Fc tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Adrenomedullin/ADM and proadrenomedullin N-20 terminal peptide/PAMP are peptide hormones that act as potent hypotensive and vasodilatator agents (PubMed:8387282, PubMed:9620797). Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, ADM is diuretic and natriuretic, and both ADM and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
- Sequence similarities
Belongs to the adrenomedullin family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The adrenomedullin (ADM) protein alternatively known as protein n20 is a peptide hormone with a mass of approximately 6 kilodaltons. It is mainly expressed in the adrenal medulla but you can also find it in various tissues including the heart lungs and kidneys. ADM is involved in multiple physiological processes operating as a vasodilator and playing a role in blood pressure regulation. The presence of ADM in endothelial and smooth muscle cells emphasizes its role in vascular function.
- Biological function summary
Adrenomedullin contributes to homeostasis and cellular growth. It is not a part of a complex but interacts with specific receptors like the calcitonin receptor-like receptor (CRLR) and receptor activity-modifying proteins (RAMPs). These interactions activate signaling cascades that contribute to mitogenesis and angiogenesis highlighting ADM's role in cellular protection and tissue repair. ADM's activity extends to immune modulation and fluid-electrolyte balance.
- Pathways
Adrenomedullin shows involvement in signaling pathways like the cAMP and MAPK pathways. It interacts closely with proteins such as RAMP2 and RAMP3 which define its receptor heterodimers in a tissue-dependent manner. ADM influences cellular responses by promoting vasodilation and modulating inflammatory responses fitting integrally into vascular homeostasis and immune functions.
- Associated diseases and disorders
ADM relates to cardiovascular and inflammatory diseases. It shows altered expression levels in conditions such as heart failure and sepsis. In these diseases ADM interacts with molecules like endothelin-1 which affect vascular tone and endothelial function. Understanding ADM's role can lead to potential therapeutic strategies targeting its pathways in cardiovascular and inflammatory pathologies.
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SDS-PAGE - Recombinant Human Adrenomedullin/ADM protein (Fc Chimera) (ab276417)
SDS-PAGE analysis of ab276417
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