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AB124570

Recombinant Human ADRM1/ARM-1 protein

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Recombinant Human ADRM1/ARM-1 protein is a Human Full Length protein, in the 1 to 407 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

GP110, ADRM1, Proteasomal ubiquitin receptor ADRM1, 110 kDa cell membrane glycoprotein, Adhesion-regulating molecule 1, Proteasome regulatory particle non-ATPase 13, Rpn13 homolog, Gp110, ARM-1, hRpn13

1 Images
SDS-PAGE - Recombinant Human ADRM1/ARM-1 protein (AB124570)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADRM1/ARM-1 protein (AB124570)

15% SDS-PAGE analysis of 3 μg ab124570.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q16186

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as ADRM1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD","proteinLength":"Full Length","predictedMolecularWeight":"44.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":407,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16186","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADRM1 known also as ARM-1 or arm protein is an essential component of the proteasome system playing an important role in ubiquitin-proteasome pathway. Its molecular mass is about 42-46 kDa. ADRM1 protein is expressed across various tissues with significant presence in tissues requiring active protein degradation such as liver muscle and neurons. This protein interacts with other subunits in large protein complexes contributing to the regulation of protein turnover.
Biological function summary

The ADRM1/ARM-1 protein binds ubiquitinated substrates and shuttles them to the 26S proteasome for degradation. It acts as a receptor on the regulatory particle of the proteasome complex where it recruits deubiquitinating enzymes. This positions ADRM1 as an integral part of the protein degradation machinery ensuring removal of damaged misfolded or unnecessary proteins and maintaining cellular protein homeostasis.

Pathways

ADRM1 plays a significant role within the ubiquitin-proteasome pathway which is important for protein catabolism. It partners with regulatory proteins like Rpn13 to mediate the recognition and processing of polyubiquitinated substrates. The efficient functioning of this pathway highlights its importance in regulating the cell cycle and various signaling pathways including NF-kB signaling which affects immune response and apoptosis.

Dysfunction of ADRM1 can lead to pathological consequences. Its aberrant activity links to cancer particularly in solid tumors due to its role in controlling the degradation of cell cycle proteins. Additionally alterations in ADRM1 functionality can affect Alzheimer's disease as proper protein turnover is important in preventing accumulation of protein aggregates. Interactions with proteins like USP14 and UCHL5 further illustrate ADRM1's involvement in these pathologies marking it as a potential therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab124570 was purified using conventional chromatography techniques.

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General info

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). Within the complex, functions as a proteasomal ubiquitin receptor (PubMed : 18497817). Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation (PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 24752541). UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex (PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 24752541).

Sequence similarities

Belongs to the ADRM1 family.

Post-translational modifications

Ubiquitinated by UBE3C in response to proteotoxic stress.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair (PubMed : 16815440, PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 18497817, PubMed : 24752541, PubMed : 25702870, PubMed : 25702872). Within the complex, functions as a proteasomal ubiquitin receptor (PubMed : 18497817). Engages and activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation (PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 24752541). UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex (PubMed : 16906146, PubMed : 16990800, PubMed : 17139257, PubMed : 24752541).
See full target information ADRM1
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Product promise

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For full details, please see our Terms & Conditions
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