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AB87670

Recombinant Human ADX protein (T7 tag N-Terminus)

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(1 Publication)

Recombinant Human ADX protein (T7 tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

ADX, FDX1, Adrenal ferredoxin, Ferredoxin-1, Hepatoredoxin

1 Images
SDS-PAGE - Recombinant Human ADX protein (AB87670)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADX protein (AB87670)

15% SDS-PAGE showing ab87670 at approximately 15kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P10109

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Adrenodoxin
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Sequence info

[{"sequence":"MASMTGGQQMGRGSMSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10109","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alternate names for ADX include ADX mouse and FDX1. This target is a protein with a mass of approximately 54 kDa. ADX is expressed in various tissues with a high presence in adrenal cortex and testes. Mechanically it functions as an adrenodoxin which transfers electrons in the adrenal gland mitochondria. As a small iron-sulfur protein ADX interacts with cytochrome P450 enzymes to facilitate steroidogenic reactions.
Biological function summary

ADX plays a significant role in enabling steroid biosynthesis and metabolism acting as an electron donor. ADX is an essential component of the mitochondrial cholesterol side-chain cleavage complex. This complex helps in converting cholesterol into pregnenolone initiating steroid hormone production. It is necessary for producing corticosteroids androgens and estrogens.

Pathways

ADX holds critical functions in the cholesterol and steroid biosynthesis pathways. ADX interacts with the cholesterol transport protein StAR and cytochrome P450scc both essential for initiating the conversion of cholesterol to pregnenolone. Additionally it supports the electron transfer processes necessary for the final conversion steps of various steroid hormones.

Abnormal ADX functioning connects to disorders like congenital adrenal hyperplasia and Addison's disease. These conditions are often linked with insufficient steroid hormone production. Furthermore defects or imbalances in the ADX pathway may involve related proteins such as cytochrome P450 enzymes which could lead to disrupted steroidogenesis and further complications in adrenal gland function.
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Specifications

Form

Liquid

Additional notes

ab87670 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method).

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General info

Function

Essential for the synthesis of various steroid hormones (PubMed : 20547883, PubMed : 21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed : 20547883, PubMed : 21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed : 20547883, PubMed : 21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

Sequence similarities

Belongs to the adrenodoxin/putidaredoxin family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Essential for the synthesis of various steroid hormones (PubMed : 20547883, PubMed : 21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed : 20547883, PubMed : 21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed : 20547883, PubMed : 21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).
See full target information FDX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:2409-2425 PubMed39933533

2025

Targeting FDX1 by trilobatin to inhibit cuproptosis in doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Wei,Guozhen Lan,Wenfang Zhang,Wang Ran,Yu Wei,Xin Liu,Yuandong Zhang,Qihai Gong,Haibo Li,Jianmei Gao
View all publications
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Complementary Products

Primary Antibodies

AB108257

Anti-ADX antibody [EPR4629]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (AB108257)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Proteins & Peptides

AB289731

Recombinant Human VHL Protein

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Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

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