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Recombinant Human AFAP protein (denatured) is a Human Fragment protein, in the 250 to 590 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Alternative names=Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M G C S G P V D S E C P P P P S S P V H K A E L E K K L S S E R P S S D G E G V V E N G I T T C N G K E Q V K R K K S S K S E A K G T V S K V T G K K I T K I I S L G K K K P S T D E Q T S S A E E D V P T C G Y L N V L S N S R W R E R W C R V K D N K L I F H K D R T D L K T H I V S I P L R G C E V I P G L D C K H P L T F R L L R N G Q E V A V L E A S S S E D M G R W I G I L L A E T G S S T D P E A L H Y D Y I D V E M S A S V I Q T A K Q T F C F M N R R V I S A N P Y L G G T S N G Y A H P S G T A L H Y D D V P C I N G S L R G K K P P V A S N G V T G K G K T L S S Q P K K A D P A A V V K R T G S N A A Q Y K Y G K N R V E A D A K R L Q T K E E E L L K R K E A L R N R L A Q L R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human AFAP protein (denatured) is a Human Fragment protein, in the 250 to 590 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Alternative names=Actin filament-associated protein 1, 110 kDa actin filament-associated protein, AFAP-110, AFAP1, AFAP
Key facts
- Purity
>80% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
39.5 kDa
- Amino acids
250 to 590
- Sequence
M G S S H H H H H H S S G L V P R G S H M G C S G P V D S E C P P P P S S P V H K A E L E K K L S S E R P S S D G E G V V E N G I T T C N G K E Q V K R K K S S K S E A K G T V S K V T G K K I T K I I S L G K K K P S T D E Q T S S A E E D V P T C G Y L N V L S N S R W R E R W C R V K D N K L I F H K D R T D L K T H I V S I P L R G C E V I P G L D C K H P L T F R L L R N G Q E V A V L E A S S S E D M G R W I G I L L A E T G S S T D P E A L H Y D Y I D V E M S A S V I Q T A K Q T F C F M N R R V I S A N P Y L G G T S N G Y A H P S G T A L H Y D D V P C I N G S L R G K K P P V A S N G V T G K G K T L S S Q P K K A D P A A V V K R T G S N A A Q Y K Y G K N R V E A D A K R L Q T K E E E L L K R K E A L R N R L A Q L R K
- Accession
- Q8N556-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Specifications
- Form
Liquid
- Additional notes
ab181922 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
- Post-translational modifications
Phosphorylated on tyrosine residues by SRC.
- Subcellular localisation
Cytoskeleton, Stress fiber
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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