Recombinant Human AGT Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
SDS-PAGE, sELISA
No
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.Angiotensin-2Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed:10619573, PubMed:17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed:1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).Angiotensin-3Stimulates aldosterone release.Angiotensin 1-7Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
SERPINA8, AGT, Angiotensinogen, Serpin A8
Recombinant Human AGT Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293, with >=80% purity and suitable for SDS-PAGE, sELISA.
>=80% SDS-PAGE
HEK 293 cells
His tag C-Terminus
SDS-PAGE, sELISA
No
Yes
Human
pH: 7.3 - 7.5
Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic
Full Length
51.8 kDa
Recombinant
His tag C-Terminus
Liquid
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Belongs to the serpin family.
Beta-decarboxylation of Asp-25 in angiotensin-2, by mononuclear leukocytes produces alanine (PubMed:17138938). The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor (PubMed:17138938).
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
This supplementary information is collated from multiple sources and compiled automatically.
Angiotensin is a peptide hormone critical in regulating blood pressure and fluid balance. Often referred to as Angiotensin I II or III depending on its form this target has a variable mass depending on the specific peptide variant. It primarily expresses in the liver where it is originally synthesized as a precursor protein called angiotensinogen. This protein circulates in the bloodstream and is cleaved to form active peptides when needed. Angiotensin acts on vascular smooth muscles and adrenal glands playing a significant role in vasoconstriction and aldosterone secretion.
The peptide interacts with specific receptors in different tissues to exert its effects. It is part of the renin-angiotensin system (RAS) where it forms a functional complex with enzymes such as renin and angiotensin-converting enzyme (ACE). This system includes various feedback mechanisms maintaining homeostasis within the cardiovascular and renal systems. Angiotensin II the most active form binds to receptors on blood vessels increasing their constriction and therefore raising blood pressure. It also influences sodium and water retention through its action on the kidneys.
This peptide fits into the larger framework of the RAS which is important for the regulation of vascular resistance. Angiotensin interacts directly with angiotensinogen which renin processes to form Angiotensin I. Subsequently ACE converts Angiotensin I to Angiotensin II. This cascade affects several downstream pathways influencing cardiovascular function and fluid retention. By impacting other proteins involved in these pathways such as aldosterone it plays an integral role in circulatory and renal physiology.
Angiotensin is closely linked to hypertension and heart failure. Dysregulation of the renin-angiotensin system or overproduction of Angiotensin II often leads to elevated blood pressure contributing to these conditions. Angiotensin receptor blockers (ARBs) and ACE inhibitors serve as therapeutic interventions in these diseases. Moreover in the case of heart failure excessive activity of Angiotensin can worsen the condition by increasing cardiac workload. It interacts with other proteins like ACE in these disorders forming a target for therapeutic modulation to improve patient outcomes.
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SDS-PAGE analysis of ab316410
Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.
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