Recombinant Human AK3 protein is a Human Fragment protein, in the 1 to 223 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S M A S K L L R A V I L G P P G S G K G T V S Q R I A Q N F G L Q H L S S G H F L R E N I K A S T E V G E M A K Q Y I E K S L L V P D H V I T R L M M S E L E N R R G Q H W L L D G F P R T L G Q A E A L D K I C E V D L V I S L N I P F E T L K D R L S R R W I H P P S G R V Y N L D F N P P H V H G I D D V T G E P L V Q Q E D D K P E A V A A R L R Q Y K D V A K P V I E L Y K S R G V L H Q F S G T E T N K I W P Y V Y T L F S N K I T P I Q S K E A Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity (PubMed:11485571, PubMed:32822537). Could also use ITP as phosphate donor (PubMed:11485571). Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).
Alternative names
AK3L1, AK6, AKL3L, AK3, Adenylate kinase 3, Adenylate kinase 3 alpha-like 1, Adenylate kinase isozyme 3
Recommended products
Recombinant Human AK3 protein is a Human Fragment protein, in the 1 to 223 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9UIJ7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S M A S K L L R A V I L G P P G S G K G T V S Q R I A Q N F G L Q H L S S G H F L R E N I K A S T E V G E M A K Q Y I E K S L L V P D H V I T R L M M S E L E N R R G Q H W L L D G F P R T L G Q A E A L D K I C E V D L V I S L N I P F E T L K D R L S R R W I H P P S G R V Y N L D F N P P H V H G I D D V T G E P L V Q Q E D D K P E A V A A R L R Q Y K D V A K P V I E L Y K S R G V L H Q F S G T E T N K I W P Y V Y T L F S N K I T P I Q S K E A Y
- Accession
- Q9UIJ7
- Protein length
- Fragment
- Amino acids
- 1 to 223
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified by using conventional chromatography techniques.
General info
- Function
Mitochondrial adenylate kinase with a specific GTP:AMP phosphotransferase activity (PubMed:11485571, PubMed:32822537). Could also use ITP as phosphate donor (PubMed:11485571). Its physiological function is to recycle GTP into GDP which is necessary for the TCA cycle in the mitochondrial matrix (Probable).
- Sequence similarities
Belongs to the adenylate kinase family. AK3 subfamily.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AK3 also known as adenylate kinase 3 is a mitochondrial enzyme involved in energy metabolism. It has a molecular mass of approximately 26 kDa and is mainly found in the mitochondrial matrix. AK3 catalyzes the reversible transfer of phosphate groups among adenosine phosphates which plays a role in cellular energy homeostasis. Expression of AK3 occurs in tissues with high metabolic rates such as the heart liver and muscle reflecting its importance in energy demand areas.
- Biological function summary
Adenylate kinase 3 facilitates the maintenance of cellular energy balance by ensuring the proper concentration of adenine nucleotides within the mitochondria. This enzyme is an important component of the adenine nucleotide translocase complex which regulates nucleotide transport across the inner mitochondrial membrane. Through its activity AK3 contributes to the recycling of ATP and ADP supporting overall energy metabolism and mitochondrial function.
- Pathways
Adenylate kinase 3 plays a significant role in the purine nucleotide cycle and oxidative phosphorylation pathways. In the purine nucleotide cycle it interacts with enzymes like nucleoside diphosphate kinase for maintaining proper levels of ADP and ATP. Within oxidative phosphorylation AK3 helps in balancing energy supply in concert with other mitochondrial proteins like ATP synthase ensuring efficient ATP production and utilization in energy-intensive processes.
- Associated diseases and disorders
Alterations in adenylate kinase 3 function have been linked to mitochondrial diseases and cardiac disorders. Mitochondrial dysfunction resulting from impaired AK3 activity can lead to energy deficiency contributing to diseases like Leigh syndrome. Similarly inadequate AK3 function may affect cardiac energy metabolism relating to heart failure conditions where proper energy supply is essential. AK3's interactions with proteins like creatine kinase further emphasize its role in maintaining cardiac energy stability.
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1 product image
SDS-PAGE - Recombinant Human AK3 protein (ab78680)
ab78680 on 15% SDS-PAGE.
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