Recombinant Human AKR1B10 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A T F V E L S T K A K M P I V G L G T W K S P L G K V K E A V K V A I D A G Y R H I D C A Y V Y Q N E H E V G E A I Q E K I Q E K A V K R E D L F I V S K L W P T F F E R P L V R K A F E K T L K D L K L S Y L D V Y L I H W P Q G F K S G D D L F P K D D K G N A I G G K A T F L D A W E A M E E L V D E G L V K A L G V S N F S H F Q I E K L L N K P G L K Y K P V T N Q V E C H P Y L T Q E K L I Q Y C H S K G I T V T A Y S P L G S P D R P W A K P E D P S L L E D P K I K E I A A K H K K T A A Q V L I R F H I Q R N V I V I P K S V T P A R I V E N I Q V F D F K L S D E E M A T I L S F N R N W R A C N V L Q S S H L E D Y P F D A E Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:12732097, PubMed:18087047, PubMed:19013440, PubMed:19563777, PubMed:9565553). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097).
Alternative names
AKR1B11, AKR1B10, Aldo-keto reductase family 1 member B10, ARL-1, Aldose reductase-like, Aldose reductase-related protein, Small intestine reductase, ARP, hARP, SI reductase
Recommended products
Recombinant Human AKR1B10 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O60218-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M A T F V E L S T K A K M P I V G L G T W K S P L G K V K E A V K V A I D A G Y R H I D C A Y V Y Q N E H E V G E A I Q E K I Q E K A V K R E D L F I V S K L W P T F F E R P L V R K A F E K T L K D L K L S Y L D V Y L I H W P Q G F K S G D D L F P K D D K G N A I G G K A T F L D A W E A M E E L V D E G L V K A L G V S N F S H F Q I E K L L N K P G L K Y K P V T N Q V E C H P Y L T Q E K L I Q Y C H S K G I T V T A Y S P L G S P D R P W A K P E D P S L L E D P K I K E I A A K H K K T A A Q V L I R F H I Q R N V I V I P K S V T P A R I V E N I Q V F D F K L S D E E M A T I L S F N R N W R A C N V L Q S S H L E D Y P F D A E Y
- Accession
- O60218
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab85415 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:12732097, PubMed:18087047, PubMed:19013440, PubMed:19563777, PubMed:9565553). Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:18087047). Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:19013440, PubMed:19563777). Displays no reductase activity towards glucose (PubMed:12732097).
- Sequence similarities
Belongs to the aldo/keto reductase family.
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldo-keto reductase family 1 member B10 (AKR1B10) also known as ARL-1 (aldo-keto reductase-like-1) is an enzyme encoded by the AKR1B10 gene. The enzyme has a molecular weight of approximately 36 kDa. It is mainly expressed in the liver gastrointestinal tract and to a lesser extent in the lung and adrenal gland. As an enzyme AKR1B10 catalyzes the reduction of carbonyl groups to their corresponding alcohols using NADPH as a cofactor. This function plays a role in the detoxification of aldehydes and ketones.
- Biological function summary
AKR1B10 protects cells from disorders caused by toxic aldehydes. It is important in lipid biosynthesis through the reduction of retinaldehyde to retinol which is part of the retinoic acid metabolism. AKR1B10 forms part of the aldo-keto reductase superfamily consisting of multiple related enzymes that regulate various cellular processes. This enzyme class is involved in stabilizing the oxidoreduction balance within cells.
- Pathways
AKR1B10 engages in lipid metabolism and the protection against oxidative stress. It is an important player in the retinoid metabolism and signaling pathway where it collaborates with enzymes like retinaldehyde dehydrogenase. Additionally AKR1B10 is involved in the polyol pathway although its exact role here continues to be studied. The enzyme's relationship with NADPH suggests it could intersect with systems managing cellular responses to oxidative stress.
- Associated diseases and disorders
AKR1B10 is associated with certain cancers and complications related to chronic alcohol consumption. Overexpression of AKR1B10 has been linked to hepatocellular carcinoma where it possibly collaborates with proteins like the carcinogenic beta-catenin pathway. Furthermore it plays a part in promoting resistance to chemotherapeutic drugs due to its detoxification role. In liver diseases caused by alcohol AKR1B10 helps protect hepatocytes from oxidative and aldehyde stress with aldehyde dehydrogenase being another involved enzyme in these processes.
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1 product image
SDS-PAGE - Recombinant Human AKR1B10 protein (ab85415)
15% SDS-PAGE showing ab85415 at approximately 36kDa (3μg).
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