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AB151381

Recombinant Human AKR1C2 protein

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Recombinant Human AKR1C2 protein is a Human Full Length protein, in the 1 to 323 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

DDH2, AKR1C2, Aldo-keto reductase family 1 member C2, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, Dihydrodiol dehydrogenase/bile acid-binding protein, Type III 3-alpha-hydroxysteroid dehydrogenase, DD-2, DD2, DD/BABP

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P52895

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Supplied as a 0.2 µM filtered solution.
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Sequence info

[{"sequence":"MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEAVKLAIEAGFHHIDSAHVYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWSNSHRPELVRPALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEAMEKCKDAGLAKSIGVSNFNHRLLEMILNKPGLKYKPVCNQVECHPYFNQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKRTPALIA","proteinLength":"Full Length","predictedMolecularWeight":"28.81 kDa","actualMolecularWeight":null,"aminoAcidEnd":323,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P52895","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 6 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR1C2 also known as Aldo-keto reductase family 1 member C2 is an enzyme with a molecular mass of approximately 37 kDa. It performs an important role in the reduction of ketosteroids. This protein transforms steroids prostaglandins and other carbonyl-containing compounds into their respective alcohol products employing NADPH as a cofactor. AKR1C2 is mostly expressed in liver tissues but it is also found in the prostate mammary glands and some brain regions.
Biological function summary

AKR1C2 is involved in drug metabolism and hormone regulation. This enzyme contributes to converting circulating hormones such as androgens and estrogens into their active forms thereby influencing hormonal balance. While not directly part of a larger protein complex AKR1C2 interacts with other enzymes within the aldo-keto reductase family which have overlapping substrates and contribute to maintaining steroid homeostasis.

Pathways

Steroid metabolism and prostaglandin pathways prominently feature AKR1C2. The enzyme plays a role in the conversion of dihydrotestosterone (DHT) to a less active form impacting androgen signaling. Additionally AKR1C2 relates to the arachidonic acid pathway by modulating prostaglandin availability with interactions seen with enzymes like microsomal prostaglandin E synthase-1 (mPGES-1) showing its broader involvement in inflammatory response.

AKR1C2 connects significantly to prostate cancer and polycystic ovary syndrome (PCOS). The enzyme's activity influences androgen levels with excessive or diminished activity linked to disease progression. In prostate cancer its modulation of DHT may contribute to cancer cell proliferation often seen with other dysregulated proteins in the androgen receptor pathway. Similarly in PCOS altered AKR1C2 expression can disrupt normal hormonal balances with implications on reproductive health.
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Specifications

Form

Liquid

Additional notes

Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.Supplied as a 0.2 µM filtered solution.

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General info

Function

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed : 19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed : 14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed : 10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 15929998, PubMed : 17034817, PubMed : 17442338, PubMed : 8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed : 10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed : 19218247). Displays affinity for bile acids (PubMed : 8486699).

Sequence similarities

Belongs to the aldo/keto reductase family.

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Product protocols

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Target data

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed : 19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed : 14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed : 10998348). Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 15929998, PubMed : 17034817, PubMed : 17442338, PubMed : 8573067). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT (PubMed : 10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed : 19218247). Displays affinity for bile acids (PubMed : 8486699).
See full target information AKR1C2
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