Recombinant human AKR1C4 protein (His tag N-Terminus)
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Recombinant human AKR1C4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 343 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, FuncS.
View Alternative Names
CHDR, AKR1C4, Aldo-keto reductase family 1 member C4, 3-alpha-hydroxysteroid dehydrogenase type I, 3alpha-hydroxysteroid 3-dehydrogenase, Chlordecone reductase, Dihydrodiol dehydrogenase 4, HAKRA, 3-alpha-HSD1, CDR, DD-4, DD4
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human AKR1C4 protein (His tag N-Terminus) (AB109831)
15% SDS-PAGE image, using ab109831 at 3ug
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AKR1C4 functions to reduce ketosteroids into their respective alcohol forms. This activity is important in steroid metabolism and detoxification processes. AKR1C4 is not part of any major enzyme complexes but its function is essential for processing steroids within cells. It impacts hormonal activity by converting potent steroids into their less active forms facilitating the regulation of physiological responses.
Pathways
AKR1C4 is part of the steroid hormone metabolic pathway and the bile acid biosynthesis pathway. In steroid hormone metabolism it interacts closely with proteins such as AKR1C3 which also acts on similar substrates. Its activity in bile acid biosynthesis ensures the proper conversion and excretion of bile acids essential for lipid digestion and absorption.
Specifications
Form
Liquid
Additional notes
ab109831 was purified using conventional chromatography.
General info
Function
Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as an NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain (PubMed : 10634139, PubMed : 10998348, PubMed : 11158055, PubMed : 14672942, PubMed : 1530633, PubMed : 19218247, PubMed : 7650035). Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH (PubMed : 14672942). Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity (PubMed : 14672942). Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol) (PubMed : 10998348, PubMed : 11158055, PubMed : 14672942). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency (PubMed : 14672942). Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids (PubMed : 10998348, PubMed : 14672942, PubMed : 19218247). Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route (PubMed : 2427522).
Sequence similarities
Belongs to the aldo/keto reductase family.
Post-translational modifications
The N-terminus is blocked.
Target data
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