JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB93634

Recombinant Human AKR7A2 protein

5

(1 Review)

|

(0 Publication)

Recombinant Human AKR7A2 protein is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

AFAR, AFAR1, AKR7, AKR7A2, Aflatoxin B1 aldehyde reductase member 2, AFB1 aldehyde reductase 1, Aldoketoreductase 7, Succinic semialdehyde reductase, AFB1-AR 1, SSA reductase

1 Images
SDS-PAGE - Recombinant Human AKR7A2 protein (AB93634)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human AKR7A2 protein (AB93634)

15% SDS-PAGE showing ab93634 at approximately 44kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

O43488

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSELEMLSAASRVVSRAAVHCALRSPPPEARALAMSRPPPPRVASVLGTMEMGRRMDAPASAAAVRAFLERGHTELDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYLHAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKALQAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLVAHECPNYFR","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43488","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AKR7A2 also known as aflatoxin aldehyde reductase or succinic semialdehyde reductase is an enzyme with a molecular mass of approximately 38 kDa. It is part of the aldo-keto reductase superfamily and catalyzes the reduction of aldehydes and ketones to their corresponding alcohols using NAD(P)H as a cofactor. AKR7A2 is expressed in various tissues with significant levels in the liver kidney and lungs indicating its involvement in detoxification processes.
Biological function summary

AKR7A2 participates in the detoxification of harmful aldehydes and builds up resistance against oxidative stress. This enzymatic activity helps in converting reactive aldehydes which are formed during lipid peroxidation into less harmful compounds. AKR7A2 does not form part of a larger enzymatic complex but interacts with other molecules within the cell to fulfill its protective roles. By metabolizing these aldehydes AKR7A2 contributes to safeguarding cellular integrity and function.

Pathways

AKR7A2 is involved in cellular defense mechanisms and detoxification pathways. It plays a role in the glutathione metabolism pathway important for handling oxidative damage in cells. AKR7A2 also intersects with pathobiological pathways related to aldehyde detoxification. Its activity correlates with proteins such as glutathione S-transferases (GSTs) which together help neutralize and excrete toxic substances from the body maintaining cellular health.

AKR7A2 has links to cancer and neurodegenerative diseases. High levels of this enzyme are associated with increased capacity to detoxify carcinogenic aldehydes which could contribute to resistance against chemotherapy in certain cancers. Additionally alterations in AKR7A2 activity are seen in neurodegenerative disorders due to its pivotal role in protecting neurons from oxidative stress. Proteins like aldehyde dehydrogenases (ALDHs) also participate in these oxidative pathways and have connections with AKR7A2 in such disease contexts highlighting the enzyme's importance in disease prevention and progression.
style
left-column

Specifications

Form

Liquid

Additional notes

ab93634 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

Sequence similarities

Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.
See full target information AKR7A2
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0
Proteins & Peptides

AB153791

Recombinant Human VMO1 protein

SDS-PAGE - Recombinant Human VMO1 protein (AB153791)

  • 0
  • 0
Proteins & Peptides

AB151884

Recombinant Human Vasorin protein

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com